FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373842
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG23440.6270028514720123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16460.440292957987599No Hit
GTGCTGGTGCACACACCAATTTATGCCTACAGCCTCCTAGTACAAACACC15350.41060127005526403No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13770.3683374259713997No Hit
CTTTATACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAA12390.3314234355690372No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11650.3116289769474805No Hit
CTTTTTACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAA11440.30601163058190356No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10780.2883571134329476No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10740.28728714269664724No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10700.2862171719603469No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10160.271772567020292No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG9370.25064064497835986No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG8970.23994093761535623No Hit
CAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG8930.23887096687905587No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8790.23512606930200458No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8450.22603131804345153No Hit
GCTACTCTGTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAG8370.2238913765708508No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8010.21426163994414751No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7550.20195697647669336No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7550.20195697647669336No Hit
CCTGTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG7440.19901455695186734No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7410.19821207889964212No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7350.19660712279519155No Hit
GTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG7240.19366470327036556No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7030.18804735690478866No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7020.18777986422071355No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6960.18617490811626303No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT6900.18456995201181248No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6630.17734764954178503No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAGTACAAACAAC6610.17681266417363486No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6520.17440523001695904No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC6450.1725327812284334No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6430.17199779586028321No Hit
CTCTAAGGCCTCCCGATATAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6380.17066033243990775No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6360.17012534707175758No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6330.1693228690195323No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6210.16611295681063123No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6180.16531047875840596No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5870.15701820555207815No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5810.1554132494476276No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5780.15461077139540233No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5750.15380829334317705No Hit
ACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5660.15140085918650126No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG5450.14578351282092436No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5410.14471354208462398No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTTTTATGT5320.14230610792794815No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5300.14177112255979799No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5290.1415036298757229No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCTT5210.13936368840312216No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5190.138828703034972No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC5150.13775873229867164No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5130.13722374693052147No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5110.13668876156237125No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4880.1305364298286442No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT4870.13026893714456908No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4870.13026893714456908No Hit
ATATAGAGCAGAGGCGGTGTCGAGGAGATCTCGAACAGAAGGAAAGAAGT4850.12973395177641892No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4840.12946645909234383No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA4760.1273265176197431No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4750.127059024935668No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTCTGTC4730.12652403956751784No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4730.12652403956751784No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4710.12598905419936765No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4710.12598905419936765No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4610.12331412735861674No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4560.12197666393824129No Hit
CACCTGTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4550.12170917125416619No Hit
CGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAACA4460.11930173709749038No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4450.11903424441341529No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACT4440.1187667517293402No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4420.11823176636119002No Hit
GGGTGTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4370.11689430294081456No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4360.11662681025673947No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGCTTTAGTACAATATGTT4280.11448686878413876No Hit
ATATTGTACTAAAGCTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA4270.11421937610006365No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4250.11368439073191347No Hit
CTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACAT4240.11341689804783839No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACT4230.1131494053637633No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4220.11288191267968821No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4190.11207943462746295No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAAT4180.11181194194338785No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4170.11154444925931274No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4140.11074197120708748No Hit
TGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCAT4050.10833453705041167No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTTTTATGTAAGACCTTGGG3990.10672958094596113No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT3970.10619459557781095No Hit
GGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGG3960.10592710289373587No Hit
GTCTTACATAAAAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3950.10565961020966076No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT3940.10539211752558567No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3940.10539211752558567No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3920.10485713215743549No Hit
CTCCACAGAAGCTCCAAATTCTTTATACGGGTCAATGTCCATGCCCCAAA3890.10405465410521023No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3850.10298468336890985No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3840.10271719068483476No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3840.10271719068483476No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3820.10218220531668458No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT3810.1019147126326095No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT3800.1016472199485344No Hit
CCTTTTTACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGA3750.10030975652815896No Hit
GCTAGGAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG3750.10030975652815896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)