FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512445
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG35210.687098127603938No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT24510.47829523168340016No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG24060.46951380148113453No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG19480.380138356311409No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18890.3686249256017719No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA17130.3342797763662442No Hit
GTGTGTAGTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTA16710.32608377484412965No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16170.3155460586014109No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15440.3013006273844022No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT15430.30110548449101854No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14960.29193376850198555No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13760.26851662129594395No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT13670.2667603352554908No Hit
GTGTAGTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAA13610.2655894778951888No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA12620.24627033145020444No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12570.245294616983286No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC12270.2394403301817756No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA11930.23280547180673047No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11840.23104918576627737No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA11820.23065889997951No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11540.22519489896476697No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT11400.22246289845739542No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11260.2197308979500239No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC11230.21914546926987286No Hit
GTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATACAATCTAAGCAG10890.21251061089482773No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT10750.2097786103874562No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT10510.2050951809462479No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC10260.20021660861165588No Hit
GGGTAAAGGTGCAGATATTGTTGATACAGAAAGGCCTTGTAAGTTGGCGA10230.19963117993150484No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10030.19572832206383126No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA9850.19221574998292498No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG9650.18831289211525137No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC9120.17797031876591635No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8990.1754334611519285No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8970.17504317536516115No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA8740.1705548888173365No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8400.16392003044229136No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8380.163529744655524No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGGCCAA8270.16138317282830353No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8230.1606026012547688No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT8200.16001717257461776No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8120.15845602942754833No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7950.15513860024002576No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7910.15435802866649104No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7650.14928431343851536No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7600.14830859897159696No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG7500.14635717003776014No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7430.1449911697840744No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7330.1430397408502376No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7320.14284459795685392No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7280.14206402638331916No Hit
ATACCACATCATCCATATAACTGAAAGCCAAGCAGTGGGGGAAAGCCCTG7090.13835631140902926No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA7090.13835631140902926No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6930.13523402511489038No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6900.13464859643473934No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6780.13230688171413518No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6770.1321117388207515No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA6750.13172145303398414No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6730.13133116724721677No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6730.13133116724721677No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6610.12898945252661262No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6500.12684288069939215No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA6410.125086594658939No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT6380.12450116597878796No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6280.12254973704495116No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA6270.12235459415156748No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6260.1221594512581838No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAAT6240.12176916547141645No Hit
TTCCCCCACTGCTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6190.12079345100449804No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6170.12040316521773069No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6110.11923230785742861No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA5980.11669545024344076No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5970.11650030735005708No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5950.11611002156328971No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5920.11552459288313868No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTACTAG5910.115329449989755No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5870.11454887841622027No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5870.11454887841622027No Hit
CTTTACCACAGGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAA5870.11454887841622027No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTT5860.1143537355228366No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5820.11357316394930188No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5790.11298773526915085No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5670.11064602054854668No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5660.110450877655163No Hit
GGTCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG5600.10928002029486092No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5560.10849944872132619No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5510.10752373425440778No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5490.10713344846764042No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG5430.10596259110733834No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC5410.10557230532057099No Hit
ACTACACACAGCGCTTCATTTTGCGGGTCACCATATTCTTGGGAACAAGA5400.10537716242718731No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA5400.10537716242718731No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5390.10518201953380363No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5370.10479173374703626No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5340.10420630506688523No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5300.1034257334933505No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5220.10186459034628108No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5220.10186459034628108No Hit
GTATACATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAAC5210.1016694474528974No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5160.10069373298597897No Hit
GCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT5140.10030344719921162No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5140.10030344719921162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)