FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492369
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16690.3389734122172598No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT15570.3162262449504335No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15430.31338284904208025No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15370.31216425079564314No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC13360.27134120953999946No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13040.26484201889233483No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCCATGAA12830.2605769250298049No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA11670.23701735893202047No Hit
ATGTAAGACCTTGGCCAGGTTCCGGCGGGCGTTCACGGTGGTCTCCATGC11310.22970576945339777No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT11050.22442517705217022No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10080.20472450540143672No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10070.2045214056936972No Hit
ACCTCATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTG9980.2026935083240415No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9550.19396022089124212No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGGCCCACAATTCGTTGACATACT9510.19314782206028405No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA9480.1925385229370655No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9140.1856331328739218No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG9010.18299283667330804No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8790.17852464310303856No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG8350.16958825596249966No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8060.16369836443805358No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7730.1569960740826494No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA7560.15354337905107754No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG7420.15069998314272426No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA7230.14684108869567336No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7010.1423728951254039No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6970.14156049629444584No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6940.14095119717122728No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC6940.14095119717122728No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAACGGCACTAGTAAACTGAG6910.1403418980480087No Hit
CTTTTATATAAAATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT6870.13952949921705063No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGATAGTACGACAGAAT6860.1393263995093111No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6850.1391232998015716No Hit
GGGTAAAGGTTCAGATACAGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6740.13688920301643687No Hit
TTATACCGCTGTTACCAATTTTCTTTTATCTTTGGGTATACATTTAAACC6500.13201481003068835No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6470.1314055109074698No Hit
CGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTC6430.13059311207651172No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCT6410.1301869126610327No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6350.12896831441459555No Hit
GCGGCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCAC6350.12896831441459555No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAAC6320.128359015291377No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6270.12734351675267938No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC6200.12592181879850273No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6180.12551561938302372No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6160.12510941996754465No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGGCCCACAATTCG6130.1245001208443261No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6070.12328152259788898No Hit
ACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTG6020.12226602405919137No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5980.1214536252282333No Hit
TTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGA5970.12125052552049377No Hit
TTCCTAAAACATAATTTGATTTTTTGTACAATATGTTCCTGTGGCAAGGA5960.12104742581275425No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGC5830.11840712961214048No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5820.11820402990440097No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG5790.1175947307811824No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAA5780.11739163107344287No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA5720.11617303282700575No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5710.11596993311926625No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5700.11576683341152673No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5670.11515753428830817No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCA5660.11495443458056863No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5620.11414203574961056No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT5580.11332963691865247No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5570.11312653721091295No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG5500.1117048392567363No Hit
CTTTAATCCTGAGTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG5490.11150173954899678No Hit
CTATTGTGGTTTCACATTTCTTGTCTTACTTTTGGAAGAGAAACTGTTCT5470.11109554013351775No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5450.11068934071803871No Hit
GGGTTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAC5430.11028314130255967No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGCCAGGTTCCGGCGGGCGTTC5420.11008004159482015No Hit
GTACAATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCC5410.10987694188708062No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5390.10947074247160159No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5300.1076428451019459No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5260.10683044627098781No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCTTG5260.10683044627098781No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5240.10642424685550878No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5230.10622114714776926No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA5160.1047994491935926No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG5140.10439324977811358No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5120.10398705036263453No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5100.10358085094715548No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAG5090.10337775123941598No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5080.10317465153167643No Hit
CTCTTTGGAAGGCGGGTATTTTATATAAAAGAGAGACAACACGTAGCGCC5070.10297155182393693No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG5020.10195605328523931No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGGCCCACAAT4980.10114365445428124No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAGGAGACTA4940.10033125562332315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)