FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492369
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16950.3442540046184874No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT14710.2987596700848348No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14430.29307287826812817No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC13540.27499700427931084No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12980.2636234206458977No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12890.261795523276242No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCCATGAA12450.2528591361357031No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT11430.23214296594627198No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA11000.2234096785134726No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10040.20391210657047862No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9540.19375712118350263No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG9140.1856331328739218No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA8710.17689984544112242No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8670.17608744661016434No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA8510.172837851286332No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA8340.16938515625476014No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGGCCCACAATTCGTTGACATACT8290.16836965771606255No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG8180.16613556093092782No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC8060.16369836443805358No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7980.1620735667761374No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7870.15983946999100268No Hit
ATGTAAGACCTTGGCCAGGTTCCGGCGGGCGTTCACGGTGGTCTCCATGC7670.15577747583621226No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG7410.15049688343498474No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7290.14805968694211047No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7160.14541939074149673No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA6990.14196669570992487No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC6820.13851400067835304No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAAC6790.13790470155513448No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT6780.13770160184739494No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCT6690.13587370447773925No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6650.13506130564678118No Hit
CTTTTATATAAAATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT6550.13303030856938597No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6550.13303030856938597No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGATAGTACGACAGAAT6530.13262410915390693No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAACGGCACTAGTAAACTGAG6400.12998381295329317No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6330.12856211499911652No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6320.128359015291377No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6320.128359015291377No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6260.1271404170449399No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGCCT6250.12693731733720034No Hit
TTATACCGCTGTTACCAATTTTCTTTTATCTTTGGGTATACATTTAAACC6200.12592181879850273No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC6170.12531251967528417No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6080.1234846223056285No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6020.12226602405919137No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG6020.12226602405919137No Hit
GGGTAAAGGTTCAGATACAGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5920.12023502698179617No Hit
CGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTC5900.11982882756631713No Hit
GCGGCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCAC5890.1196257278585776No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5860.11901642873535904No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5780.11739163107344287No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5770.11718853136570337No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5770.11718853136570337No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5590.113532736626392No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAA5540.1125172380876944No Hit
TTCCTAAAACATAATTTGATTTTTTGTACAATATGTTCCTGTGGCAAGGA5540.1125172380876944No Hit
CTTTAATCCTGAGTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG5540.1125172380876944No Hit
GTACAATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCC5540.1125172380876944No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCA5510.11190793896447583No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT5450.11068934071803871No Hit
CTATTGTGGTTTCACATTTCTTGTCTTACTTTTGGAAGAGAAACTGTTCT5420.11008004159482015No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA5410.10987694188708062No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5370.10906454305612255No Hit
GCGCCTGCGCGGAACCTTTGTGTCTCCTCTGCCGATCCATACTGCGGAAC5350.1086583436406435No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5260.10683044627098781No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5260.10683044627098781No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGGCCCACAATTCG5250.10662734656324831No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5220.10601804744002973No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG5210.10581494773229021No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5170.10500254890133214No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5150.10459634948585309No Hit
TTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGA5100.10358085094715548No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5050.10256535240845788No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5010.1017529535774998No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCTTG5000.10154985386976029No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG4980.10114365445428124No Hit
GGGTTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAC4980.10114365445428124No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAGGAGACTA4960.1007374550388022No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4960.1007374550388022No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG4940.10033125562332315No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4930.10012815591558363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)