FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352885
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12190.34543831559856614No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10550.2989642518100798No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8740.247672754580104No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7910.22415234424812616No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7620.21593436955382067No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7550.21395072048967795No Hit
GGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATATTTAGTAACATT7120.201765447667087No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7070.20034855547841363No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6740.19099706703316943No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6680.18929679640676142No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6670.18901341796902674No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT6550.18561287671621068No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6440.18249571390112926No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6250.17711152358417048No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6070.17201071170494636No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5960.16889354888986496No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5920.1677600351389263No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5850.16577638607478357No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5760.1632259801351715No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5730.1623758448219675No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5500.15585814075407003No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATA5480.15529138387860067No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5420.15359111325219263No Hit
CACCTATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT5390.1527409779389886No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5190.14707340918429518No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4960.1405557051163977No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4960.1405557051163977No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4960.1405557051163977No Hit
ATGCAGTTGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA4900.13885543448998966No Hit
CTAAATAATCAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGG4890.13857205605225498No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4590.1300707029202148No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4560.12922056760701078No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4530.12837043229380676No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4510.12780367541833743No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4450.12610340479192939No Hit
GATTCTTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTT4400.12468651260325603No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4340.12298624197684797No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4290.12156934978817462No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4260.12071921447497061No Hit
CTCTAAGGCCTCCCGATACAGAGCGGAGGCGGTGTCGAGAAGATCTCGAA4220.11958570072403191No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4160.11788543009762387No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4150.1176020516598892No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4120.11675191634668516No Hit
CCACCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4110.11646853790895052No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4110.11646853790895052No Hit
GTCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA4110.11646853790895052No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4070.11533502415801182No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4050.11476826728254247No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4020.11391813196933846No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3990.11306799665613443No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA3880.10995083384105304No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT3860.1093840769655837No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT3810.10796718477691034No Hit
ATCCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA3800.10768380633917565No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3790.10740042790144098No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3780.1071170494637063No Hit
TCCTCAGGCCATGCAGTGGAACACCACCACTTTCCACCAAACTCTTCAAG3770.10683367102597163No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3770.10683367102597163No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3760.10655029258823695No Hit
GTATAAAGAATTTGGAGCTTCCGTGGAGTTACTCTCTTTTTTGCCTTCTG3750.10626691415050228No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCCGTGGAGTTACTCTCTTTT3740.10598353571276763No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3720.10541677883729828No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3710.10513340039956359No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3670.10399988664862492No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3660.10371650821089023No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3660.10371650821089023No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC3620.10258299445995153No Hit
GTGCTGGTGAACACACCTATTTATGCCTACAGCCTCCTAGTACAAAGACC3620.10258299445995153No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3610.10229961602221686No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCCGTGG3580.10144948070901286No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC3570.10116610227127817No Hit
GTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGAT3550.10059934539580884No Hit
ACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATATTTAGTA3530.10003258852033947No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC3530.10003258852033947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)