FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511355
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT28990.5669251302910894No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA22150.433162871195158No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG21600.42240713398715174No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA21060.411846955637473No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG19090.37332186054697813No Hit
GTGTTATACATGCTCGTTATAGCCTAACTACACGTCTGTTTGAATTTGGG15850.30996079044890534No Hit
GTATAACACACTGCAATTATGCAGATATTTCTGTCAAAGAGATGTCATTT14100.27573799024161294No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA12380.24210186660930275No Hit
GTTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAG11880.23232392369293345No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT11860.2319328059762787No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA11120.2174614504600522No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT11120.2174614504600522No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG11080.21667921502674267No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10830.21179024356855805No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10730.20983465498528422No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT10640.2080746252603377No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10490.20514124238542697No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT10370.20279453608549833No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT9940.19438550517742076No Hit
GATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGAGACTA9730.19027876915254568No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG9710.18988765143589093No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC9610.18793206285261707No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9390.1836297679694146No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT9240.1806963850945038No Hit
TTTGCTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACAC9150.17893635536955735No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8920.1744385016280275No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8870.17346070733639057No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA8820.17248291304475363No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT8640.1689628535948607No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8540.16700726501158686No Hit
CCATTATAACTTGTTAAACAGTGAACTTATAGTTGAATGTGATCTATTAG8500.1662250295782773No Hit
GTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8290.16211829355340224No Hit
CCTGTCGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC7900.15449149807863422No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7880.15410038036197943No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7870.15390482150365206No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7790.15234035063703297No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA7330.14334464315397327No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7270.14217129000400897No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7090.13865123055411602No Hit
CCTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC6920.13532672996255046No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6910.1351311711042231No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6860.13415337681258618No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6770.13239334708763972No Hit
CTATTAGATGGCTGCTTAACATTTGCTCGAGAATAATCTATTAGTCATCT6680.13063331736269324No Hit
GTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTG6530.12769993448778247No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6370.12457099275454431No Hit
ATTAATAGTCAGATATGTCAACGTTAATATGGGCCTAAAAATCAGACAAC6120.11968202129635967No Hit
GGGTAAAGGTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6060.11850866814639535No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6040.11811755042974059No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5940.11616196184646672No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGT5930.11596640298813934No Hit
GCTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAG5910.11557528527148458No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5870.11479304983817505No Hit
CGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA5860.11459749097984767No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5760.1126419023965738No Hit
TGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGA5750.11244634353824642No Hit
AAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGATAATA5730.11205522582159165No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5680.11107743152995472No Hit
GTACAATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5640.11029519609664518No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5430.1061884600717701No Hit
CTTCCCCGATCATCAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATC5420.10599290121344272No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5380.10521066578013318No Hit
GGACATTGTTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATG5370.1050151069218058No Hit
GATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGC5370.1050151069218058No Hit
GTATTCACACTATGATGTCTGCATGTGTGTTATGCAGACAGAAAAAAAAA5310.10384175377184147No Hit
ATCTATTAGTCATCTAAGCACAAAGCAGCTATTTGAAAATTTGTTGAGTA5310.10384175377184147No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTGT5240.10247284176354979No Hit
CTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTA5230.1022772829052224No Hit
TTGTTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAAC5220.10208172404689501No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5190.10149504747191285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)