FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787013
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN109171.387143541466278No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGG59830.7602161590723406No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT39550.5025329950077063No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG39430.5010082425576198No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA27040.343577552086179No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC23640.3003762326670589No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG23270.2956749126126252No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG22570.2867805233204534No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCA21750.276361381578195No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAA21650.2750907545364562No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT20980.26657755335680605No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT20370.2588267284021992No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA19170.24357920390133325No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT18740.2381155076218563No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT18730.23798844491768242No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTG18650.23697194328429136No Hit
ATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG16740.21270296678707976No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT16710.21232177867455812No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT16180.20558745535334233No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA15950.202665013157343No Hit
GATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC15510.1970742541736922No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC15390.19554950172360558No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAACTATT15340.19491418820273618No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA14400.1829702940103912No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAA13080.16619801705943868No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACAGACT12810.16276732404674382No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12720.16162375970917886No Hit
GTTTGGAAGTAATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCC12640.16060725807578782No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG12290.15616006342970193No Hit
ATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA11900.1512046179669205No Hit
ATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA11700.14866336388344284No Hit
ATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAG11630.14777392495422567No Hit
CCAATCAATAGGTCTATTTAAAGGAATTTTCCGAAAACATTGTTTGATTT11460.14561385898326967No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11440.14535973357492188No Hit
CAATAGGTCTATTTAAAGGAATTTTCCGAAAACATTGTTTGATTTTGTGT11240.14281847949144422No Hit
ATTTAAAGGAATTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT11060.14053135081631432No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC11020.14002309999961882No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA11010.13989603729544492No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10720.13621121887440232No Hit
ACATCGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT10570.1343052783117941No Hit
GATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTA10190.12947689555318653No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA10160.1290957074406649No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT10040.1275709549905783No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGGAT9880.12553795172379617No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC9780.12426732468205734No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9630.12236138411944912No Hit
TTATTATCCTGAACACACAGTTAATCATTACTTCCAAACTAGGCATTATT9630.12236138411944912No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9600.12198019600692747No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC9360.11893069110675428No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9120.1158811862065811No Hit
GGTCTATTTAAAGGAATTTTCCGAAAACATTGTTTGATTTTGTGTACAAT9050.11499174727736391No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9040.11486468457319002No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA9000.11435643375649449No Hit
CAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATT8920.11333993212310343No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT8780.11156105426466907No Hit
GGGTATACATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTA8750.11117986615214744No Hit
TAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA8720.11079867803962579No Hit
GTTTCTCTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATG8630.10965511370206082No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG8550.10863861206866977No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT8500.10800329854780036No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8360.106224420689366No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8160.10368316660588833No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC8070.1025396022683234No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG7910.10050659900154126No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA7900.1003795362973674No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7880.10012541088901962No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7880.10012541088901962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)