FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306850
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG27970.9115202867850742No Hit
ATCCCAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT23620.7697572103633696No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT22120.7208733909076096No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAA22060.7189180381293792No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC21730.7081635978491119No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA19030.6201727228287437No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT18750.6110477431970017No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG17800.5800879908750204No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG12010.3913964477757862No Hit
CATCTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT11880.38715985008962034No Hit
TCGCCAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGCC11530.375753625549943No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC11460.3734723806420075No Hit
CTATCAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG11320.36890989082613657No Hit
GTAATTCGTTGACAGACTTTCCAATCAATAGGTCTATTTATAGGAAGTTT11140.36304383249144534No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG10910.3555483135082288No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG10760.35065993156265274No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9960.32458856118624735No Hit
CTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT9470.30861984683069904No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8540.27831187876812774No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC8220.2678833306175656No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT7890.25712889033729835No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7850.2558253218184781No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7490.24409320514909563No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA7320.2385530389441095No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7240.23594590190646894No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT7200.2346423333876487No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6840.22291021671826625No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6750.21997718755092066No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6410.20889685514094833No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6250.20368258106566728No Hit
ATCTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCATG6110.19912009124979632No Hit
TCCACAAAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5800.1890174352289392No Hit
CTTTCCCGATCACCAGTTGGACCTTGCATTCAGAGCCAATTCAAACAATC5720.18641029819129867No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5710.1860844060615936No Hit
GGTTGGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG5570.18152191624572267No Hit
TGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGG5520.17989245559719733No Hit
CTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT5430.1769594264298517No Hit
GTAATTCGTTGACAGACTTTCCAATCAATAGGTCTATTTACAGGAAGTTT5360.17467818152191625No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5350.17435228939221117No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5150.16783444679810983No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5100.16620498614958448No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5020.16359784911194394No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4980.16229428059312367No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG4970.1619683884634186No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCGTAAGTCGTTGACAGACT4810.15675411438813752No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4750.15479876160990713No Hit
CTTATATAAGACCTTGGGCAACACCTGTCGGGCGTTCACGGTGGTCTCCA4720.1538210852207919No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG4700.1531693009613818No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4600.1499103796643311No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4380.1427407528108196No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4370.14241486068111456No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4330.1411112921622943No Hit
CATGTATACAAGCTAAGAAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4300.14013361577317907No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4280.13948183151376894No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC4270.13915593938406387No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATG4190.13654880234642333No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA4190.13654880234642333No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4160.13557112595730814No Hit
CCTATAAATAGACCTATTGATTGGAAAGTCTGTCAACGACTTACGGGGCT4110.1339416653087828No Hit
CTTTCCCGGTCACCAGTTGGACCTTGCATTCAGAGCCAATTCAAACAATC4100.13361577317907772No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGA4060.13231220466025745No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC4010.13068274401173213No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG3980.1297050676226169No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA3910.12742382271468145No Hit
ATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAG3900.12709793058497637No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC3900.12709793058497637No Hit
TTATTGATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAA3890.1267720384552713No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTC3880.12644614632556622No Hit
ATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTTGGA3880.12644614632556622No Hit
TATTGATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA3880.12644614632556622No Hit
CCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAATTGG3860.1257943620661561No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT3790.12351311715822064No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3790.12351311715822064No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG3730.12155776437999023No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG3700.12058008799087502No Hit
ATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA3600.11732116669382435No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3600.11732116669382435No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3510.11438813752647874No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3500.11406224539677366No Hit
ATCGCCAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGC3480.11341046113736353No Hit
GTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA3420.11145510835913312No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT3410.11112921622942805No Hit
ACGACTTACGGGGCTTCTGGGCTTTGCCGCTCCCTTTACACAATGTGGTT3400.110803324099723No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT3400.110803324099723No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3370.1098256477106078No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3360.10949975558090272No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3230.10526315789473684No Hit
GGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTCAAATGAATG3190.10395958937591658No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAGATGTATA3170.10330780511650643No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3170.10330780511650643No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3120.1016783444679811No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3100.10102656020857097No Hit
ACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA3100.10102656020857097No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA3080.10037477594916082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)