FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545009
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG19020.3489850626319932No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT17560.32219651418600426No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA16780.30788482392033895No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16650.3054995422093947No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT16410.3010959452045746No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15970.2930226840290711No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA15480.28403200681089674No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA15420.2829311075596917No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA14480.2656836859574796No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT14480.2656836859574796No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA13950.25595907590516853No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC13840.2539407606112926No Hit
GTACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGCAACTCTATG13570.24898671398087005No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTTTTCCCTAAACTTCAT13030.2390786207200248No Hit
GTATATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG12070.2214642327007444No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT11860.21761108532152681No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA10690.1961435499230288No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATTATTAATTTCAATCT10680.19596006671449462No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTTTTCCCTAA10470.19210691933527702No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC10450.1917399529182087No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG10440.19155646970967452No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC10220.18751983912192277No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT9890.1814648932402951No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA9830.1803639939890901No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC9690.17779522906961168No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG9630.17669432981840666No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG9560.17540994735866747No Hit
GTATACATGCATACAAGGGCATTAAGGCAGGATATCCACATTGTGTAAAT9540.17504298094159912No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9480.1739420816903941No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT9440.17320814885625743No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA9210.16898803505997148No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC9060.16623578693195892No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG9010.16531837088928808No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG8820.1618321899271388No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8410.15430937837723782No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT8340.15302499591749863No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA8190.15027274778948604No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT8110.14880488212121268No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC8000.1467865668273368No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTAT7890.14476825153346093No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT7720.14164903698838No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC7700.14128207057131165No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7610.13963072169450413No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG7300.13394274222994482No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7230.13265835977020562No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7210.13229139335313728No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA7060.12953914522512472No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT7050.12935566201659054No Hit
GGATAGAATCTAGCAGGCATTATTAATTTCAATCTTCTCTTTTCATTTAC6960.127704313139783No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG6900.126603413888578No Hit
CTCTAAGGCTTCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGA6760.12403464896909959No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6680.12256678330082621No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6590.1209154344240187No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC6530.11981453517281367No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT6530.11981453517281367No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG6510.11944756875574532No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA6430.11797970308747195No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT6420.11779621987893778No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT6400.11742925346186943No Hit
TCCCTAAACTTCATGGGTTACATAATTGGAAGTTGGGGAACATTGCCACA6180.11339262287411768No Hit
GCCGCAGTCCCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGA6170.11320913966558352No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6160.11302565645704933No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6120.11229172362291265No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATTA6110.11210824041437849No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6100.1119247572058443No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6080.11155779078877598No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT6050.11100734116317346No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACC5980.10972295870343425No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAAGTAGAGGACAAACGGGCAAC5970.10953947549490009No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5940.10898902586929757No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5930.10880554266076341No Hit
GTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT5870.10770464340955838No Hit
TCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGACGGAAGGAAA5850.10733767699249003No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC5840.10715419378395587No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT5810.10660374415835336No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5790.10623677774128501No Hit
CTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGT5710.10476891207301163No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5610.10293407998766993No Hit
TGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCATA5560.10201666394499906No Hit
CCTCTGCACGTTGCATGGAGACCACCGTGAACGCCCATCAGATCCTGCCC5530.10146621431939655No Hit
ATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATACAAGCTAAAC5480.1005487982767257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)