FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545009
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA17660.324031346271346No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG17610.32311393022867513No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA16890.3099031392142148No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT16630.3051325757923264No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16540.3034812269155188No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT16390.30072897878750626No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA15390.2823806579340892No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA15330.28127975868288413No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13820.2535737941942243No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT13510.247885814729665No Hit
GTATATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG13000.23852817109442231No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA12940.2374272718432173No Hit
GTACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGCAACTCTATG12750.23394109088106801No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC12720.23339064125546552No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTTTTCCCTAAACTTCAT12530.22990446029331627No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT10950.20091411334491727No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG10840.19889579805104138No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA10790.1979783820083705No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTTTTCCCTAA10480.1922904025438112No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG10290.18880422158166196No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA10100.1853180406195127No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT9810.17999702757202174No Hit
GTATACATGCATACAAGGGCATTAAGGCAGGATATCCACATTGTGTAAAT9760.1790796115293509No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC9700.17797871227814588No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATTATTAATTTCAATCT9610.17632736340133834No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG9580.17577691377573582No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC9550.17522646415013332No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA9430.17302466564772326No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT9300.17063938393677902No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT9200.1688045518514373No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG9130.16752016939169811No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC9130.16752016939169811No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG9060.16623578693195892No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG8480.155593760836977No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG8350.15320847912603278No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT8310.1524745462918961No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA8110.14880488212121268No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8070.148070949287076No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC8010.14697005003587096No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7950.14586915078466595No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT7860.1442178019078584No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT7510.13779588960916242No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC7450.1366949903579574No Hit
GGATAGAATCTAGCAGGCATTATTAATTTCAATCTTCTCTTTTCATTTAC7370.135227124689684No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7360.13504364148114986No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG7290.13375925902141067No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7110.1304565612677956No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT7060.12953914522512472No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7040.12917217880805637No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA7030.1289886955995222No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATTA7020.12880521239098805No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6820.12513554822030462No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTAT6720.1233007161349629No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6630.12164936725815537No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC6540.11999801838134784No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG6460.11853015271307446No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC6310.11577790458506189No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6290.11541093816799357No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT6250.11467700533385687No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA6080.11155779078877598No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6070.11137430758024179No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG6050.11100734116317346No Hit
GTATTAACATAATTGACTACTAGATCTCTGGATGCTGGATCTTCCAAATT6020.11045689153757095No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACC6010.11027340832903676No Hit
TCCCTAAACTTCATGGGTTACATAATTGGAAGTTGGGGAACATTGCCACA5980.10972295870343425No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5970.10953947549490009No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT5960.10935599228636592No Hit
ATATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG5910.10843857624369506No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5750.10550284490714833No Hit
CTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGT5720.10495239528154582No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT5710.10476891207301163No Hit
GTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT5700.10458542886447747No Hit
ATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATACAAGCTAAAC5640.10348452961327244No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5630.10330104640473828No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC5610.10293407998766993No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5600.10275059677913577No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5520.10128273111086239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)