FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634832
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA43330.6825427829725029No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40450.6371764498323965No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT25660.4042014265191421No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC24140.3802580840285304No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA19470.30669531466592737No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT17370.27361569675126646No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT16390.2581785417244247No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT16110.25376792600246995No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT15640.2463643924691887No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT15410.242741386697583No Hit
CACTAATATGGGACTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT14170.22320865992892608No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC14130.2225785719686468No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT13980.22021574211759962No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC13510.21281220858431837No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG13220.20824407087229377No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13210.20808654888222394No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT13160.2072989389318749No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC13140.20698389495173528No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13020.20509363107089748No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT12540.19753257554754644No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA12440.1959573556468483No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12300.1937520477858709No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT11990.18886886609370668No Hit
ACCTAGTAGTTAGTTATGTCAACACTAATATGGGACTAAAGTTCAGGCAA11830.18634851425258966No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG11830.18634851425258966No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA11320.17831489275902918No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC11090.17469188698742344No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT11050.1740617990271442No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG10930.17217153514630643No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAATCTCCTCC10670.16807596340449127No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT10670.16807596340449127No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG10630.16744587544421202No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA10630.16744587544421202No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT10060.15846712201023264No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAATCT10040.158152078030093No Hit
GTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA9990.15736446807974394No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACGTAGCGCC9750.15358394031806838No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG9480.14933084658618345No Hit
ATATTAGTGTTGACATAACTAACTACTAGGTCCCTTGATGCTGGATCTTC9380.1477556266854853No Hit
CTAATATGGGACTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTTGT9360.14744058270534566No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9350.14728306071527586No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT9100.1433450109635305No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT9020.14208483504297198No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT9000.14176979106283238No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA8970.1412972250926229No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8840.13924943922171534No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA8700.13704413136073795No Hit
TCCTAGTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGT8700.13704413136073795No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA8210.1293255538473171No Hit
CTCTAAGGCTTCCCGATAGAGAGCTGAGGCGGTATCTAGAAGATCTCGTA8210.1293255538473171No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8170.12869546588703784No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT8060.12696272399626987No Hit
CTCACAGTCAATGAAAAACGAAGACTACAATTAATTATGCCTGCTAGGTT8030.12649015802606045No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7980.12570254807571138No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA7930.12491493812536231No Hit
CACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGT7920.1247574161352925No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA7880.12412732817501324No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7820.12318219623459435No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG7780.12255210827431509No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT7670.12081936638354714No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7670.12081936638354714No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA7580.11940166847291883No Hit
AATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT7480.11782644857222069No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCAT7460.11751140459208105No Hit
CTACAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTT7400.11656627265166217No Hit
CAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCC7340.11562114071124328No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA7330.11546361872117347No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC7300.11499105275096404No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7290.11483353076089421No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA7110.11199813493963758No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG7060.1112105249892885No Hit
GACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG6930.10916273911838092No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG6930.10916273911838092No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6910.1088476951382413No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA6880.10837512916803185No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6830.10758751921768278No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG6750.10632734329712429No Hit
TAATTGTAGTCTTCGTTTTTCATTGACTGTGAGTGGGCCTACAAATTGTT6630.10443707941628652No Hit
TTGACAGACTTTCCAATCAATTGGCCTGTTAACAGGAAGTTTTCTAAAAC6620.1042795574262167No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA6580.10364946946593745No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6560.10333442548579781No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA6500.10238929354537893No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6480.1020742495652393No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA6410.10097159563475062No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6350.10002646369433174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)