FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698879
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT42870.6134109051781497No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36270.5189739568652084No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT35470.5075270540393975No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGTGTTT27250.38991012750418885No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA22690.3246627813970659No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG22460.32137179683464523No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTC21500.30763551344367196No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT20810.2977625597564099No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT18370.2628495061376862No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA18300.26184790214042775No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT17450.24968556788800353No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG16540.23666471592364344No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT15650.22393003652992866No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA15620.22350077767396073No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT15620.22350077767396073No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA15610.2233576913886381No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG14730.21076609828024595No Hit
CTTTAATCCTGAATGGCAAACACCTTCTTTTCCTAAAATTCATTTACATG14350.20532881943798567No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA13720.1963143834626595No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTT13220.18916006919652759No Hit
GGATCATATTGTACACAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA12970.1855829120634616No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT12530.17928711550926554No Hit
CATACTTTCCAATCAATAGGTCTATTGACAGGAAGTTTTCTAAAACAGTG11460.16397688297974328No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC11460.16397688297974328No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC11410.1632614515531301No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGTGTTTGCCATTCAGGAT11140.15939812184941887No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT10810.1546762744337718No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG10720.15338849786586806No Hit
CTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGGTTTTAT10670.15267306643925485No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC10670.15267306643925485No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10520.1505267721594153No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA10510.15038368587409265No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA10390.14866665045022098No Hit
CCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA10220.14623418359973614No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTCCTAAAATTCA9990.14294319903731548No Hit
GAAATTAATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGC9900.14165542246941173No Hit
CTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGG9750.13950912818957217No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG9540.13650431619779677No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG9430.13493036705924774No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT9400.13450110820327982No Hit
CTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTCCT9150.13092395107021387No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG9090.13006543335827803No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT9080.1299223470729554No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT9060.12963617450231013No Hit
TTCTAAAACAGTGTTTGATTTTGTGTACAATATGATCCTGTGGCAATGTA8760.12534358594263098No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA8750.12520049965730834No Hit
GAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCA8680.12419889566004988No Hit
CAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATG8570.12262494652150087No Hit
GCACAAAATATTTGCCTTTAGACAAAGGGATTAAACCTTATTATCCTGAT8540.12219568766553295No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA8480.12133716995359713No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATATT8450.1209079110976292No Hit
ATATTTGCCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAG8450.1209079110976292No Hit
GGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTT8410.12033556595633865No Hit
AATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGCCTTTAG8290.118618530532467No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8280.11847544424714435No Hit
CATATTTTCCAATCAATAGGTCTATTGACAGGAAGTTTTCTAAAACAGTG8230.11776001282053115No Hit
TTGTAGGCCCTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATG8200.11733075396456326No Hit
GGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAA8180.11704458139391798No Hit
TATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAACCTTA8050.11518445968472367No Hit
GGTCTATTGACAGGAAGTTTTCTAAAACAGTGTTTGATTTTGTGTACAAT7980.11418285568746522No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC7870.11260890654891619No Hit
ATGTTAATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCAT7750.11089187112504455No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT7740.11074878483972189No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT7610.10888866313052759No Hit
ATCATATTGTACACAAAATCAAACACTGTTTTAGAAAACTTCCTGTCAAT7540.10788705913326914No Hit
CAATAGGTCTATTGACAGGAAGTTTTCTAAAACAGTGTTTGATTTTGTGT7380.10559767856810692No Hit
ATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTT7340.10502533342681639No Hit
GTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAAC7270.10402372942955791No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA7130.10202052143504098No Hit
GTTTTGATGACCAACCTCCCATGATGTAGCTCTTGTTCCCAAGAATATGG7090.10144817629375041No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT7060.1010189174377825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)