FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698879
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT37880.5420108488021532No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36080.5162553174440784No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT30810.440848845079048No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGTGTTT24750.3541385561735293No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG24160.34569646533949366No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA22230.3180808122722245No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTC20900.29905033632431366No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT18390.2631356787083315No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG18380.2629925924230088No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT18370.2628495061376862No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA18100.258986176433975No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT17730.2536919838770374No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA16390.23451842164380388No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT15640.223786950244606No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG15280.21863584397299102No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT15210.21763423997573256No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA14890.21305547884540812No Hit
CTTTAATCCTGAATGGCAAACACCTTCTTTTCCTAAAATTCATTTACATG14440.20661659600588944No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA13320.19059093204975397No Hit
GGATCATATTGTACACAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA12230.17499452694958642No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC12170.17413600923765057No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTCCTAAAATTCA11790.16869873039539032No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTT11350.16240293384119425No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGTGTTTGCCATTCAGGAT11290.16154441612925843No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC11170.15982738070538677No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC10990.15725182756957928No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG10800.15453318814844916No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT10660.15252998015393224No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10600.1516714624419964No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA10480.14995442701812473No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT10350.14809430530893045No Hit
CTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGG10050.14380171674925132No Hit
CTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGGTTTTAT10040.14365863046392865No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATATT10020.1433724578932834No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT9890.1415123361840891No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA9880.14136924989876645No Hit
CCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA9760.1396522144748948No Hit
CATACTTTCCAATCAATAGGTCTATTGACAGGAAGTTTTCTAAAACAGTG9590.13721974762440994No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG9570.13693357505376466No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG9400.13450110820327982No Hit
CTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTCCT9390.13435802191795718No Hit
TTGTAGGCCCTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATG9300.13307024535005343No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG9240.13221172763811762No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA9100.13020851964360067No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA8980.12849148421972903No Hit
ATATTTGCCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAG8830.12634518993988947No Hit
GAAATTAATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGC8760.12534358594263098No Hit
TTCTAAAACAGTGTTTGATTTTGTGTACAATATGATCCTGTGGCAATGTA8600.12305420537746876No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG8570.12262494652150087No Hit
GGTCTATTGACAGGAAGTTTTCTAAAACAGTGTTTGATTTTGTGTACAAT8550.12233877395085557No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT8450.1209079110976292No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT8320.11904778938843491No Hit
GAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCA8260.11818927167649908No Hit
GTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAAC8180.11704458139391798No Hit
GGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTT8030.1148982871140784No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7970.11403976940214257No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC7960.11389668311681993No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT7960.11389668311681993No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7880.11275199283423883No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA7850.11232273397827092No Hit
GCACAAAATATTTGCCTTTAGACAAAGGGATTAAACCTTATTATCCTGAT7840.11217964769294829No Hit
CAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATG7810.11175038883698037No Hit
GTTTTGATGACCAACCTCCCATGATGTAGCTCTTGTTCCCAAGAATATGG7720.11046261226907662No Hit
GTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC7490.10717162770665595No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG7340.10502533342681639No Hit
AATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGCCTTTAG7300.10445298828552582No Hit
GGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAA7250.10373755685891264No Hit
GTTTGATGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGC7190.1028790391469768No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT7100.10159126257907306No Hit
ATGTTAATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCAT7050.10087583115245986No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT7040.10073274486713724No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTATACC7030.1005896585818146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)