FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573136
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39930.6966932804779319No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGGACAAAGACC27620.4819100527623461No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT23990.41857429999162504No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA19460.3395354680215516No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC14970.2611945506825605No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13460.23484827335920272No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC12200.21286396248010944No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12060.2104212612713213No Hit
ATTTATGCCTACAGCCTCCTAGGACAAAGACCTTTAATCTAATCTCCTCC11850.20675720945813905No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT11750.20501242288043325No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT11370.19838223388515117No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA11360.19820775522738057No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT11050.19279891683649256No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTATTCTTTACATTTTAT10510.18337706931688116No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCTTTGTATGCATGTATT10310.1798874961614695No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10200.17796823092599312No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9660.16854638340638173No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9620.1678484687752994No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9600.16749951145975825No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA9570.1669760754864465No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG9480.16540576756651126No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9440.16470785293542894No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT9230.16104380112224673No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG9030.1575542279668351No Hit
TCCTAGGACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT8820.15389017615365289No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA8780.15319226152257057No Hit
CTTATAAGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCC8760.15284330420702938No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8700.1517964322604059No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTACG8670.15127299628709415No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC8480.14795790178945312No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA8420.1469110298428296No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8090.14115323413640044No Hit
GACCAATTTATGCCTACAGCCTCCTAGGACAAAGACCTTTAATCTAATCT7690.1341740878255772No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGGCCGGCAGATGAGAAGGCACAG7660.13365065185226543No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7610.13277825856341252No Hit
GAGTTCCGCAGTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGC7230.1261480695681304No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7200.12562463359481868No Hit
GGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTAAACAATACC7100.12387984701711288No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC7070.12335641104380113No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAATGAGA7000.12213506043940706No Hit
ACATTCTCGGGACAGATAACTCTGTTGTTCTCTCCCGCAAATATACATCG6980.12178610312386588No Hit
GAATACATGCATACAAAGGCTTTAAAGCAGGATAACCACATTGTGTAAAA6970.12161162446609532No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6970.12161162446609532No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT6880.12004131654616008No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6850.11951788057284833No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6840.11934340191507775No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6820.11899444459953659No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6800.11864548728399542No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6690.11672622204851903No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCG6620.11550487144412495No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC6600.1151559141285838No Hit
CATGTATTCAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC6590.11498143547081321No Hit
TGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTT6580.11480695681304262No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6570.11463247815527206No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6480.11306217023533682No Hit
ATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC6430.1121897769464839No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG6290.10974707573769577No Hit
GCTTTAAAGCCTTTGTATGCATGTATTCAATCCAAGCAGGCTTTCACTTT6190.10800228915998995No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6180.10782781050221936No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6150.10730437452890762No Hit
TCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6130.10695541721336646No Hit
GTCATAGAGTACTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6130.10695541721336646No Hit
GTGTAAATGTATCTGAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6090.10625750258228413No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTGCACTTCGCTTCAC6080.10608302392451355No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6080.10608302392451355No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT6050.1055595879512018No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5960.10398928003126656No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCTGTCCCGAGAATGTTT5960.10398928003126656No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5950.103814801373496No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGCTTTGCTCGCAGCAGGTCTG5830.10172105748024901No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG5810.10137210016470785No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5800.10119762150693726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)