FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573136
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39820.6947740152424555No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT29780.5195974428407918No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGGACAAAGACC25190.4395117389240948No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA18090.31563189190698193No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT14110.2461893861142905No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13030.2273456910750677No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11190.1952416180452807No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11100.19367131012534547No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10940.19087965160101616No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT10930.19070517294324557No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10810.18861142904999859No Hit
ATTTATGCCTACAGCCTCCTAGGACAAAGACCTTTAATCTAATCTCCTCC10740.18739007844560454No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCTTTGTATGCATGTATT10710.1868666424722928No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10050.1753510510594344No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTATTCTTTACATTTTAT10010.1746531364283521No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC10000.1744786577705815No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9900.1727338711928757No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9860.17203595656179335No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9340.16296306635772312No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA9240.16121827978001732No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9160.15982245051785265No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA8740.15249434689148825No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8730.15231986823371765No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTGCACTTCGCTTCAC8700.1517964322604059No Hit
TCCTAGGACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT8240.14377041400295915No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT8180.14272354205633567No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT8090.14115323413640044No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTG8090.14115323413640044No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7740.13504648111443007No Hit
CTTATAAGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCC7720.13469752379888894No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTACG7690.1341740878255772No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7630.1331272158789537No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC7610.13277825856341252No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG7510.1310334719857067No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA7500.13085899332793613No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7260.12667150554144216No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7200.12562463359481868No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA7150.12475224030596577No Hit
GACCAATTTATGCCTACAGCCTCCTAGGACAAAGACCTTTAATCTAATCT7090.12370536835934229No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAATGAGA7030.1226584964127188No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6980.12178610312386588No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6960.12143714580832472No Hit
GAGTTCCGCAGTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGC6750.11777309399514252No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6670.11637726473297785No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6660.11620278607520729No Hit
GCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG6580.11480695681304262No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC6520.11376008486641913No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6230.10870020379107229No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6200.10817676781776055No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT6200.10817676781776055No Hit
GTGTAAATGTATCTGAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6100.10643198124005472No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6070.10590854526674298No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6050.1055595879512018No Hit
GAATACATGCATACAAAGGCTTTAAAGCAGGATAACCACATTGTGTAAAA5970.10416375868903716No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5950.103814801373496No Hit
AATATACATCGTTTCCATGGCTGCTAGGATGTGCTGCCAACTGGATCCTG5940.10364032271572542No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG5850.10207001479579018No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5820.10154657882247844No Hit
ATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC5760.10049970687585495No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCTGTCCCGAGAATGTTT5740.10015074956031378No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5740.10015074956031378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)