FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609080
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45760.7512970381559072No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA42700.701057332370132No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT23840.3914099954029028No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT22310.36629014251001507No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAC17140.2814080252183621No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA17090.28058711499310435No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTTTAAATTTTAT16620.27287055887568135No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT16230.26646745911867076No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG15760.25875090300124776No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT14750.2421685164510409No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT14640.24036251395547384No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC13940.22886977080186513No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT13120.21540684310763775No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT12640.20752610494516321No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12510.20539173835949298No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT12190.20013791291784327No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT12170.1998095488277402No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG11720.1924213568004203No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT11460.18815262362907995No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC11380.1868391672686675No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT11190.183719708412688No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11040.18125697773691468No Hit
CACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGG10750.17649569843041965No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACACC10560.17337623957444015No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT10480.1720627832140277No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAT10180.16713732186248112No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10130.16631641163722335No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10000.16418204505155315No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATGTT9920.16286858869114074No Hit
GTTTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTA9630.1581073093846457No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA9610.1577789452945426No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAA9230.1515400275825836No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9190.15088329940237735No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT9150.15022657122217115No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT8960.14710711236619164No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG8600.14119655874433573No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC8490.13939055624876864No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA8400.13791291784330464No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA8370.13742037170815No Hit
ATCCATAACATCCAATGACATAACCCATAAAATTTAAAGAATAACCCCAT8260.1356143692125829No Hit
GGCATAATCAATTGCAATCTTCTTTTTTCATTAACTGTGAGTGGGCCTAC8220.1349576410323767No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATT8040.13200236422144873No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT8010.13150981808629408No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC7950.13052472581598476No Hit
CTCCGAAAGACACCAAATATTCTATAACTGTTTCTCTTCCAAAAGTGAGA7880.12937545150062388No Hit
GTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTT7800.12806199514021147No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTTTCA7710.12658435673474747No Hit
AAACAAAGAGATGGGGTTATTCTTTAAATTTTATGGGTTATGTCATTGGA7630.12527090037433505No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC7470.1226439876535102No Hit
GATCCATAACATCCAATGACATAACCCATAAAATTTAAAGAATAACCCCA7460.12247980560845867No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG7410.1216588953832009No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA7380.12116634924804624No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC7290.11968871084258226No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7280.1195245287975307No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG7270.11936034675247915No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCCATGTCAACGACCGACCT7260.1191961647074276No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC7250.11903198266237604No Hit
CTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT7140.11722598016680895No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA7040.11558415971629342No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC7030.11541997767124186No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG6870.11279306495041702No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT6860.11262888290536548No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6770.11115124449990149No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATTTACACACTCTATGGA6770.11115124449990149No Hit
CAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT6750.11082288040979839No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTT6660.1093452420043344No Hit
CTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCT6640.1090168779142313No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTTTCATTAAC6630.10885269586917974No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACT6420.10540487292309714No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA6380.1047481447428909No Hit
GTTATGGATCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGT6310.10359887042753005No Hit
CATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCC6280.10310632429237539No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6250.10261377815722073No Hit
GGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATAATCAATTG6250.10261377815722073No Hit
TTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATG6230.10228541406711762No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC6220.10212123202206606No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6190.1016286858869114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)