FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424907
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16320.3840840466266736No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12860.3026544632119499No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12270.2887690718204219No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11830.2784138646809773No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11750.2765310997465328No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC11210.2638224364390325No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT11180.2631163995886158No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT10080.23722838174000427No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAACAAC9930.23369819748792087No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9790.23040335885264304No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9730.22899128515180966No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9700.22828524830139302No Hit
ACACTAATGTGGGCTTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCT9410.22146022541403176No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9200.216517967461115No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8700.20475068662083704No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT8530.2007498111351425No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT8070.18992391276208678No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8020.18874718467805895No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCAGTACG7710.18145147055708663No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7560.1779212863050032No Hit
CATTAGTGTTGACATAACTAACAACTAGGTCCCTAGATGCTGGATCTTCC7560.1779212863050032No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7360.17321437396889203No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7200.16944884410000305No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG7070.16638935108153077No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7050.16591865984791965No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6930.16309451244625295No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC6900.16238847559583627No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA6810.16027036504458622No Hit
ACTACACACCGTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGT6780.15956432819416955No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6690.1574462176429195No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6590.1550927614748639No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6360.14967981228833604No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAA6150.1447375543354193No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA6090.14332548063458592No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6060.14261944378416924No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGTAGGCAAAAACGAGA6050.1423840981673637No Hit
CTCTAAGGCTTCCCGATAGAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6000.1412073700833359No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5960.14026598761611364No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5930.13955995076569697No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5900.1388539139152803No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5900.1388539139152803No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC5750.1353237296631969No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5750.1353237296631969No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5700.1341470015791691No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5650.1329702734951413No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGACTCTA5650.1329702734951413No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT5620.13226423664472461No Hit
GTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGTATGATGTGAT5620.13226423664472461No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC5550.1306168173270857No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5530.13014612609347456No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5510.12967543485986346No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCTCCGTG5480.1289693980094468No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGA5420.1275573243086134No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG5290.12449783129014112No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5200.1223797207388911No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5170.12167368388847441No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5130.12073230142125217No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5120.12049695580444662No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA5070.11932022772041882No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5070.11932022772041882No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTA5060.11908488210361325No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4990.11743746278597435No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4950.11649608031875211No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG4850.11414262415069651No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4830.11367193291708538No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4820.11343658730027983No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4750.11178916798264091No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA4690.11037709428180756No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4680.110141748665002No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4670.10990640304819642No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4650.10943571181458531No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4620.10872967496416863No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4540.10684691002972414No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4520.10637621879611303No Hit
GTTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG4510.10614087317930747No Hit
TCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG4510.10614087317930747No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTACT4490.10567018194569636No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4480.1054348363288908No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4470.10519949071208523No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4470.10519949071208523No Hit
CCTGTATTCAATCTAGGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4420.10402276262805744No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4420.10402276262805744No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4420.10402276262805744No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA4420.10402276262805744No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4400.1035520713944463No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4360.10261068892722408No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC4340.10213999769361295No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4310.10143396084319628No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4300.1011986152263907No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGGCAGACGGAGAAGG4290.10096326960958517No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA4290.10096326960958517No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4290.10096326960958517No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTT4250.10002188714236292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)