FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424907
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA17170.4040884240551462No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14230.3348968127143116No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13200.31065621418333894No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11870.2793552471481995No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT11200.263587090822227No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT10950.257703450402088No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10920.25699741355167127No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10420.24523013271139332No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAACAAC10290.24217063969292105No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8900.20945759895694824No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8840.2080455252561149No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8810.20733948840569819No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8680.2042799953872259No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8550.20122050236875363No Hit
ACACTAATGTGGGCTTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCT8420.19816100935028136No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8330.1960428987990313No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8240.19392478824778128No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8190.19274806016375348No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT8180.1925127145469479No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8080.1901592583788923No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC8010.1885118390612534No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCAGTACG7280.17133160903444752No Hit
GTTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG7240.1703902265672253No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7220.16991953533361417No Hit
CATTAGTGTTGACATAACTAACAACTAGGTCCCTAGATGCTGGATCTTCC6940.1633298580630585No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6790.1597996738109751No Hit
ACTACACACCGTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGT6640.15626948955889172No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG6400.15062119475555827No Hit
CTCTAAGGCTTCCCGATAGAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6400.15062119475555827No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6390.15038584913875272No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT6340.14920912105472492No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC6230.14662031926986374No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA6020.141678061316947No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6000.1412073700833359No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCTCCGTG5960.14026598761611364No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGTAGGCAAAAACGAGA5810.13673580336403024No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5740.13508838404639134No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5710.13438234719597464No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5670.13344096472875241No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA5670.13344096472875241No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA5640.13273492787833574No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5520.129910780476669No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5470.1287340523926412No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5460.12849870677583564No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5450.1282633611590301No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5280.12426248567333559No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT5280.12426248567333559No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC5240.12332110320611334No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5240.12332110320611334No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5200.1223797207388911No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5180.12190902950527999No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5160.12143833827166885No Hit
GTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGTATGATGTGAT5160.12143833827166885No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5150.12120299265486331No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5080.11955557333722439No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5050.11884953648680771No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5010.11790815401958546No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC5000.1176728084027799No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4980.11720211716916878No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4960.11673142593555766No Hit
GTCCCAAACGGCCCCGAGAAGGGTCGTCCGCGGGATTCAGCGCCGACGGG4940.11626073470194655No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4920.11579004346833544No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC4900.1153193522347243No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4890.11508400661791876No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4880.11484866100111318No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4870.11461331538430762No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGACTCTA4820.11343658730027983No Hit
ACCCTTCTCGGGGCCGTTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG4820.11343658730027983No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4790.11273055044986316No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4760.11202451359944646No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4740.11155382236583535No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4710.11084778551541867No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4690.11037709428180756No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG4690.11037709428180756No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4640.10920036619777974No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4580.1077882924969464No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTT4570.10755294688014083No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4570.10755294688014083No Hit
GACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG4550.10708225564652972No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4520.10637621879611303No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTACT4510.10614087317930747No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA4490.10567018194569636No Hit
TCTCGGGGCCGTTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4380.10308138016083519No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT4310.10143396084319628No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG4280.10072792399277959No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA4280.10072792399277959No Hit
GGCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC4280.10072792399277959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)