FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508037
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26430.5202376992226944No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA21050.41433990044032226No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA17470.343872591956885No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA17190.3383611823548285No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAGCATTCTTTGATTTTCTGT12630.24860394026419336No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT12540.2468324157492466No Hit
ATATTAGTGTTGACATAACTAACCACTAGGTCCCTAGATGCTGGATCTTC12010.23640010471678244No Hit
CAATTGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT11000.21651966293793562No Hit
GCCAAAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC10980.216125990823503No Hit
TTTGACGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACAC10760.2117955975647443No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA10730.21120508939309537No Hit
GGATAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGC10590.2084493845920671No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT10200.2007727783606312No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9590.1887657788704366No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9470.18640374618384092No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9260.18227018898229855No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8860.17439674669364633No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8820.1736094024647811No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8760.17242838612148328No Hit
ATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAG8740.17203471400705067No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8690.17105053372096912No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC8590.1690821731488061No Hit
ACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACC8510.16750748469107565No Hit
GACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT8480.16691697651942675No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG8460.16652330440499413No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8280.16298025537510064No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC8060.15864986211634194No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7750.15254794434263647No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7710.15176060011377124No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7630.15018591165604078No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC7530.14821755108387774No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT7430.1462491905117147No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT7300.14369032176790275No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7240.14250930542460488No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7180.14132828908130707No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7180.14132828908130707No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7100.1397536006235766No Hit
AGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTACGTCC6960.13699789582254834No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA6860.1350295352503853No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6820.13424219102152007No Hit
GGCCAATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT6670.1312896501632755No Hit
GTTCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGC6480.1275497650761657No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6470.1273529290189494No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6230.12262886364575809No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6210.12223519153132546No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6140.12085733913081134No Hit
GCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC6100.12006999490194613No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6090.11987315884472981No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT6070.1194794867302972No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5910.11633010981483632No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5910.11633010981483632No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5910.11633010981483632No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5860.1153459295287548No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5850.1151490934715385No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5810.11436174924267326No Hit
CTTACAAGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCC5810.11436174924267326No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT5770.11357440501380806No Hit
ATATGAGTGTTGACATAACTAACCACTAGGTCCCTAGATGCTGGATCTTC5770.11357440501380806No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5770.11357440501380806No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5700.11219655261329392No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5670.11160604444164499No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5660.11140920838442869No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5610.11042502809834717No Hit
CCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTT5570.10963768386948196No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5530.10885033964061672No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5440.10707881512567No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5420.10668514301123737No Hit
GGCCTATTGATTGGAAAGTTTGTCAACGTATTGTGGGTCTTTTGGGTTTT5370.10570096272515585No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5370.10570096272515585No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5320.10471678243907433No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5280.10392943821020909No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5260.10353576609577649No Hit
CTCTAAGGCTTCCCGATAGAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5250.10333893003856018No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT5230.10294525792412758No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5220.10274842186691127No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5200.10235474975247866No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5150.10137056946639712No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5110.10058322523753191No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA5110.10058322523753191No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA5090.10018955312309931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)