FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459655
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG20480.44555155497057575No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGGAAGGAAGGAGTTT19500.4242312168909291No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC19480.4237961079505281No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG17590.38267831308263806No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16220.3528733506651728No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15490.336991874340538No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAAAG15330.3335110028173304No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA15320.33329344834712993No Hit
ATTAACAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAAAG15290.3326407849365285No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14600.3176295264926956No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC12930.28129792996921604No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA12640.2749888503334022No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA12550.27303086010159794No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT12090.263023354472376No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT11900.25888981953856693No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11720.2549738390749584No Hit
GTACGAAACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG10680.23234817417410883No Hit
GTACGAGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG10100.2197300149024812No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9980.2171193612600755No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC9960.21668425231967456No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA9810.2134209352666674No Hit
CTCCAAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG9480.20624163775005167No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG9230.20080277599503976No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG9070.19732190447183215No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG8800.19144793377641928No Hit
GTCTCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC8760.19057771589561737No Hit
CCTGTTAATAATGTCCTCTTGTAAATGAATCTTAGGGAAGGAAGGAGTTT8730.18992505248501593No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC8570.18644418096180831No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC8420.18318086390880117No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC8070.17556645745178448No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG8010.17426113063058163No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT7940.17273824933917828No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA7860.1709978135775745No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT7820.17012759569677258No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7810.1699100412265721No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC7790.16947493228617114No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7490.16294829818015688No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG7440.16186052582915447No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7360.16012009006755068No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA7190.15642166407414257No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA7160.15576900066354116No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA6960.1514179112595316No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6940.15098280231913064No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAACTAATTTCAATCT6930.15076524784893017No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA6930.15076524784893017No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6880.1496774754979278No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6870.14945992102772732No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6840.1488072576171259No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG6840.1488072576171259No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT6750.14684926738532159No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6650.14467372268331682No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6590.14336839586211397No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6580.1431508413919135No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6580.1431508413919135No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG6550.14249817798131206No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA6540.1422806235111116No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT6440.14010507880910683No Hit
GTTTCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC6410.1394524153985054No Hit
GCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAATCTT6380.13879975198790398No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA6270.13640665281569872No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC6200.1348837715242954No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6160.1340135536434935No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGAT6040.13140290000108779No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6020.13096779106068682No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA6010.13075023659048635No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5970.12988001870968444No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGGGAC5960.12966246423948397No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5950.12944490976928347No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC5940.129227355299083No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC5830.12683425612687776No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT5790.12596403824607585No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5790.12596403824607585No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5770.1255289293056749No Hit
GCCTTCCACCAAGCTCTGCAGGATCCCAGAGTCAGGGGTCTGTATTTTCC5750.12509382036527394No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5740.12487626589507347No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5640.12270072119306873No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5620.12226561225266776No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5570.12117783990166539No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG5530.12030762202086347No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5510.11987251308046253No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5510.11987251308046253No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT5480.11921984966986109No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5480.11921984966986109No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT5480.11921984966986109No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG5470.11900229519966062No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5440.11834963178905919No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5420.11791452284865822No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC5420.11791452284865822No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG5420.11791452284865822No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC5410.11769696837845775No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC5350.11639164155725489No Hit
ACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGAC5330.11595653261685394No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT5320.11573897814665347No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG5310.115521423676453No Hit
GTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5240.11399854238504967No Hit
GTACTTGAATATTTGGTCTCTTTTGGAGTGTGGATTCGCACTCCTCCAGC5230.1137809879148492No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5190.11291077003404727No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTCCCTAAG5170.11247566109364633No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT5080.11051767086184203No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5000.10877723510023822No Hit
CAGTTGGACCCTGCATTCGGAGCCAACTCAAACAATCCAGATTGGGACTT4990.10855968063003775No Hit
GCCCAGGACGATGGGATGGGAATACAGGTGCAATTTCCATCCGTAGGTTT4950.10768946274923583No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA4930.10725435380883488No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG4890.10638413592803297No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC4860.10573147251743155No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT4800.10442614569622868No Hit
CTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACT4800.10442614569622868No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC4790.10420859122602821No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC4700.10225060099422394No Hit
GGATAGAATCTAGCAGGCATAACTAATTTCAATCTTCTTTTTTCATTTAC4690.10203304652402344No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4680.10181549205382297No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT4670.1015979375836225No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAA4670.1015979375836225No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG4640.10094527417302107No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4630.1007277197028206No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAATTCCACTGCCTT4620.1005101652326201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)