FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382524
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA27240.7121121811964739No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC14620.38219824115611045No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14000.3659901078102289No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC13620.35605609059823695No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA13320.3482134454308749No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11760.3074316905605923No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT11410.2982819378653365No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC10760.2812895400027188No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC10640.27815248193577397No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9780.25567023245600273No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9670.2527945958946367No Hit
GATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC9660.2525331743890579No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9490.24808900879421947No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9110.23815499158222753No Hit
CTATGTATCCCTCCTGTTGCTGTATCAAACCTTCGGACGGAAATTGCACC8860.23161945394275912No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8440.2206397507084523No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT8440.2206397507084523No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8430.22037832920287354No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA8200.21436563457456265No Hit
ACATAGAGGTTCCTTGAGCAAGAGTCGTGCAGGTTCTGCATGGTCCCGTG7730.2020788238123621No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7650.1999874517677322No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT7580.19815750122868106No Hit
GTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7470.195281864667315No Hit
GCACGGGACCATGCAGAACCTGCACGACTCTTGCTCAAGGAACCTCTATG7400.19345191412826385No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7350.19214480660037017No Hit
GGTATATTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTC7330.19162196358921268No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7250.18953059154458282No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7220.1887463270278466No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7150.18691637648879547No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7130.18639353347763798No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7080.18508642594974434No Hit
CCTTTCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTCAGGAAGG7070.18482500444416558No Hit
CTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7050.18430216143300812No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7020.18351789691627193No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC6820.17828946680469723No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6700.1751524087377524No Hit
GTATCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6670.17436814422101618No Hit
GATACATAGAGGTTCCTTGAGCAAGAGTCGTGCAGGTTCTGCATGGTCCC6640.17358387970428No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6610.17279961518754378No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT6600.17253819368196505No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6470.1691397141094415No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6420.16783260658154783No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6350.16600265604249667No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6130.1602513829197645No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG6050.15816001087513462No Hit
CCTCTATGTATCCCTCCTGTTGCTGTATCAAACCTTCGGACGGAAATTGC6050.15816001087513462No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT6000.15685290334724097No Hit
GTCATAGGCCATCAGCGCATGCGTGGAACCTTTCCGGCTCCTCTGCCGAT5990.15659148184166222No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTA5870.1534544237747174No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5790.15136305173008752No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5770.15084020871893006No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG5720.14953310119103638No Hit
GTTGGCAAGGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAG5710.14927167968545765No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5520.14430467107946168No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5500.14378182806830422No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5480.14325898505714674No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5380.1406447700013594No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5370.14038334849578066No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5340.13959908397904447No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5250.13724629042883585No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT5170.13515491838420596No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5140.13437065386746977No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5050.13201786031726115No Hit
ATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTACGAACCACTGAACA5050.13201786031726115No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5050.13201786031726115No Hit
GAAATATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCC5010.1309721742949462No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA5010.1309721742949462No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGATACAGCAACAGGAGGGA5000.13071075278936745No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5000.13071075278936745No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4970.12992648827263126No Hit
GTCTTTAAACAAACAGTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACA4950.1294036452614738No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4880.12757369472242266No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4850.12678943020568645No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4800.12548232267779275No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4790.12522090117221404No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC4790.12522090117221404No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4790.12522090117221404No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4790.12522090117221404No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4760.12443663665547783No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTT4710.12312952912758414No Hit
CAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTG4710.12312952912758414No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4700.12286810762200542No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAA4700.12286810762200542No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGT4660.12182242159969048No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4650.12156100009411173No Hit
GCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCTCACTTTT4630.12103815708295426No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4600.12025389256621807No Hit
GCCTATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4600.12025389256621807No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4580.1197310495550606No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4540.11868536353274566No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAG4540.11868536353274566No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4540.11868536353274566No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4530.11842394202716691No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT4520.11816252052158818No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC4490.117378256004852No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC4490.117378256004852No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4400.11502546245464337No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4400.11502546245464337No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4380.1145026194434859No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC4350.11371835492674968No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4340.11345693342117096No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4310.11267266890443477No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA4250.11110413987096233No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4230.11058129685980489No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4150.10848992481517498No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC4150.10848992481517498No Hit
GTTTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG4130.10796708180401754No Hit
CTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTC4060.10613713126496638No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT3920.1024772301868641No Hit
GAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3910.10221580868128535No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGATAC3900.10195438717570662No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA3900.10195438717570662No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA3890.10169296567012788No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC3890.10169296567012788No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG3850.10064727964781295No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3850.10064727964781295No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC3830.10012443663665548No Hit
CCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTACGAACCACTGAA3830.10012443663665548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)