FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621542
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29800.4794527159870129No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC27430.4413217449504619No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC27120.4363341495828118No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG21250.3418916179437592No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19370.31164426539155843No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA16810.2704563810651573No Hit
GTTTCGATGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG15480.2490579880362067No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG15230.2450357337074566No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14850.23892190712775643No Hit
ATGTTATGTTCTCCATGTTCGTCACAGGGTCCCCAGTCCTCGCGGAGATT13780.22170665860070599No Hit
GTCAAAGAATTGTGGGTCTTTTGGGCTTTGCTGCTCCATTTACACAATGT13380.21527105167470584No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA13130.2112487973459557No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT12750.20513497076625553No Hit
GTATACTTGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT12370.19902114418655537No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA11940.1921028667411052No Hit
TTTACTGGTCTATAGGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGAC11920.19178108639480518No Hit
TCTTATTGGTTCTTCTGGATTATCAAGGTATGTTGCCCGTTTGTCCTCTA10510.16909557198065456No Hit
CTACGTCAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGG10310.16587776851765448No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC9780.15735058934070426No Hit
TTATTTACACACTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCA9770.15718969916755424No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA9540.15348922518510416No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGGACACCCGTG9480.15252388414620413No Hit
CCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGAATATGATAAAA8750.1407789015062538No Hit
GGAAACTACAGACCCCTGACTCTGGGATCCTGGTTGGCTGCTGGCCAGTG8570.1378828783895537No Hit
CTAGTAAGCTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT8340.13418240440710363No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG8230.13241261250245356No Hit
CATCTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8070.1298383697320535No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACACCCGTG7980.12839035817370348No Hit
TTATATACACACTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCA7460.12002406916990324No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7410.11921961830415322No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA7330.11793249691895319No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGCTTACTAGTG7270.11696715588005316No Hit
TCCAAAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTACC7190.11568003449485313No Hit
GTATAGATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT7170.11535825414855311No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7080.11391024259020308No Hit
GCTTACTAGTGCCCTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7060.11358846224390307No Hit
GCTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAAGGG6950.11181867033925302No Hit
CATCAAAACCTCGCAAAGGCATGGGGACGAATCTTTCTGTTCCCAACCCT6860.11037065878090298No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT6850.11020976860775297No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT6750.10860086687625294No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA6710.1079573061836529No Hit
CCTCACCATACTGCACTCAGGCAAGCCGTTCTCTGCTGGGGGGAATTGAT6610.10634840445215288No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6580.10586573393270286No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTT6500.10457861254750284No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6470.10409594202805282No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6460.1039350518549028No Hit
GTCCTCAAAACCTCGCAAAGGCATGGGGACGAATCTTTCTGTTCCCAACC6440.10361327150860279No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA6340.10200436977710275No Hit
CAGTAAACCCTGCTCCGAATATTGCATCTCACATCTCGTCAATCTCCGCG6300.10136080908450273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)