FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564475
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG34000.6023295982993047No Hit
GTCCTGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC30230.5355418751937642No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC19430.34421364985163205No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18310.32437220426059615No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18250.32330926967536205No Hit
CACTAAACCCTGCTCCGAATATTGCATCTCACATCTCGTCAATCTCCGCG17650.3126799238230214No Hit
CCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT16560.2933699455246025No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGGTCACCCGTG14540.25758448115505556No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA13500.23916028167766507No Hit
AGTTTGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT13320.23597147792196288No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13240.23455423180831747No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12710.22516497630541654No Hit
ATATAAGAGAGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT12540.22215332831391998No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA11510.20390628460073518No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT10980.19451702909783428No Hit
ATCAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG10210.18087603525399706No Hit
TATCCACACTTCCGGAAACTACTGTTGTTAGACGACGGGACCGAGGGCGG10040.17786438726250056No Hit
TCACCATACTGCACTCAGGCAAGCCGTTCTCTGCTGGGGGGAATTGATGA10000.17715576420567783No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC9940.17609282962044379No Hit
CAAGATCTACCGCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGG9700.1718410912795075No Hit
GTCTAAGGGCAAATACTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA9690.17166393551530185No Hit
GTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGGATCCCAGAGTCAGGG9490.16812082023118827No Hit
ATTACAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAACAA9440.16723504141015988No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGGTTT9420.16688072988174854No Hit
CCTATTGATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT9390.1663492625891315No Hit
TACTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT9090.16103458966296114No Hit
CACTAAGTATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC8960.15873156472828734No Hit
CTATATAAGAGAGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA8910.15784578590725898No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT8680.15377120333052838No Hit
ACACTAAACCCTGCTCCGAATATTGCATCTCACATCTCGTCAATCTCCGC8590.15217680145267728No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA8580.15199964568847157No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC8510.15075955533903185No Hit
GTATTCTATATAAGAGAGAAACCACACGTAGCGCATCATTTTGCGGGTCA8430.14934230922538644No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG8430.14934230922538644No Hit
GTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAGATACAGACCCCTGA8310.14721644005491827No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7960.14101598830771955No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT7910.14013020948669117No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7830.13871296337304573No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTACC7810.13835865184463442No Hit
GTATAAAGAATTTGGAGCTACTGCTGAGTTACTCTCGTTTTTGCCTTCTG7800.1381814960804287No Hit
GGTGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGAAT7760.137472873023606No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTA7720.1367642499667833No Hit
CCTCAAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT7680.1360556269099606No Hit
GTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGATGCGCTACGT7630.1351698480889322No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7610.13481553656052084No Hit
CTTCAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7580.13428406926790382No Hit
GCCTTAGAGTCTCCTGAGCATTGCACATATCACCATACTGCACTCAGGCA7470.13233535586164136No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTACTGCCAACTGGATTCTT7390.13091810974799592No Hit
ATGTAAATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGGTTT7360.1303866424553789No Hit
GCCTAGTCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC7190.12737499446388237No Hit
CTTCTAGACACCGCCTCAGCTCTGTTTCGAGAAGCCTTAGAGTCTCCTGA7160.12684352717126532No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7050.12489481376500287No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA7010.12418619070818017No Hit
GATTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA6850.12135169848088932No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA6770.1199344523672439No Hit
GAGAAACTGTACTTGAATATTTGGTCTCTTTTGGAGTGTGGATTCGCACT6660.11798573896098144No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT6580.11656849284733602No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA6510.11532840249789628No Hit
GGAATTCCACTGCCTTCCACCAAGCTCTGCAGGATCCCAGAGTCAGGGGT6500.1151512467336906No Hit
TATCACCATACTGCACTCAGGCAAGCCGTTCTCTGCTGGGGGGAATTGAT6490.11497409096948492No Hit
ATTCTATCCTACCCACACTAAGTATTTGCCCTTAGACAAAGGAATTAAAC6490.11497409096948492No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6480.11479693520527925No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6480.11479693520527925No Hit
GTACTGAAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6440.11408831214845654No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC6420.11373400062004517No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG6410.1135568448558395No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCTAACCAGACATTATT6410.1135568448558395No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATACTTAGTGTGGGT6370.1128482217990168No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6360.1126710660348111No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGGGAC6280.11125381992116569No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGAGAAACCACACGTAGCGCA6200.10983657380752025No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6160.10912795075069756No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6150.10895079498649186No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG6110.10824217192966915No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTTCAG6090.10788786040125782No Hit
ACGTCATCAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC6070.10753354887284645No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC6050.1071792373444351No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCGCCATGC6030.10682492581602374No Hit
ATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGGGACCGAGG6020.10664777005181807No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5970.10576199123078966No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGGTTTGC5950.1054076797023783No Hit
AGTTGGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGCACTGCGAAT5950.1054076797023783No Hit
GTGTAGATAAGATAGGGGCATTTGGTGGTCTATAGGCTGGAGGAGTGCGA5890.10434474511714426No Hit
AGATAGGGGCATTTGGTGGTCTATAGGCTGGAGGAGTGCGAATCCACACT5890.10434474511714426No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5870.10399043358873289No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5860.10381327782452722No Hit
CTCCCGTCCCCTTCTCCGTCTGCCGTTCCAGCCGACCACGGGGCGCACCT5860.10381327782452722No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA5780.1023960317108818No Hit
CTCCAAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG5780.1023960317108818No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5750.10186456441826476No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATGGACATTGA5740.10168740865405908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)