FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471920
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA34020.7208848957450416No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA30820.6530767926767249No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC27390.5803949822003729No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC22670.48037803017460584No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG17550.3718850652652992No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACATACT14050.29771995253432787No Hit
CTACTGTACCTGTCTTTAACCCTTATTGGAAAACACCCTCTTTTCCTAAT13530.28670113578572637No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA12810.2714443125953551No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12800.27123241227326667No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC12680.26868960840820477No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA11910.2523732836073911No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA11340.2402949652483472No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC11080.23478555687404648No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG10450.2214358365824716No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT10370.21974063400576369No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT9810.20787421596880828No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT9660.20469571113748095No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT9260.19621969825394134No Hit
CCCATTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTT9250.19600779793185283No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8840.18731988472622477No Hit
TCCTCAGGCCATGCAGTGGAACTCAACAACCTTCCACCAAACTCTGCAAG8640.183081878284455No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTG8600.18223427699610104No Hit
ATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGC8480.17969147313103914No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG8390.17778437023224275No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8380.17757246991015427No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC8360.1771486692659773No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC8330.1765129682997118No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA8260.1750296660450924No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8070.17100355992541108No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8040.1703678589591456No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC7990.16930835734870317No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7960.1686726563824377No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7900.16740125444990678No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7890.16718935412781827No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7780.16485845058484488No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7710.16337514833022546No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7650.16210374639769454No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7630.16167994575351755No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7600.16104424478725207No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7400.1568062383454823No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC7090.1502373283607391No Hit
GTGTAAACAATACATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7080.15002542803865063No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT7040.14917782675029667No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTGCACTCAGGCA7030.14896592642820816No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6970.14769452449567724No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATAAGGGTTAAAGA6890.14599932191896933No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6850.14515172063061535No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6850.14515172063061535No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTG6760.14324461773181896No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6740.142820817087642No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6700.141973215799288No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGTG6690.14176131547719953No Hit
TCTTGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC6610.1400661129004916No Hit
CCTCATCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAGTTAAT6560.13900661129004915No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT6440.1364638074249873No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6440.1364638074249873No Hit
GATGAGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGG6430.1362519071028988No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6410.13582810645872181No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6310.1337091032378369No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT6280.13307340227157147No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6270.13286150194948296No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG6210.131590100016952No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6190.13116629937277505No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT6150.1303186980844211No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCTCATCCCATCATCC6120.12968299711815562No Hit
GTCCCGTGCTGGTGTTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6070.12862349550771318No Hit
CCTAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACG6060.12841159518562467No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6000.12714019325309373No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6000.12714019325309373No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5980.12671639260891676No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5890.12480928971012036No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5880.12459738938803187No Hit
GAATACAGGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5870.12438548906594338No Hit
GGTGTTTGAAGATCCTGGAATTAGAGGACAAACGGGCAACATACCTTGAT5830.12353788777758942No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT5780.12247838616714697No Hit
TCATCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAGTTAATGA5760.12205458552297No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5530.11718087811493473No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAGTTAATGACTCTA5500.11654517714866927No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5500.11654517714866927No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5480.1161213765044923No Hit
GTCTTTAACCCTTATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5470.1159094761824038No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5450.1154856755382268No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5390.11421427360569587No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAAACACC5370.1137904729615189No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5360.11357857263943041No Hit
GGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG5300.11230717070689947No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5280.11188337006272249No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATGTATTGTTTACAC5280.11188337006272249No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5230.11082386845228005No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATTCAATCTAAGC5200.11018816748601457No Hit
GTTTAAATGTATACCCAAAGACAACAGAAAATTGGTAACAGCGGTAAAAA5190.1099762671639261No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5170.1095524665197491No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5140.10891676555348363No Hit
ACATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTG5140.10891676555348363No Hit
GACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG5140.10891676555348363No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT5110.10828106458721817No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA5060.10722156297677574No Hit
TTGTATGCCTGTATTCAATCTAAGCAGGCTTTTTCTTTCTCGCCAACTTA5040.10679776233259874No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5020.10637396168842177No Hit
GTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA5010.10616206136633327No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC5000.10595016104424479No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4990.1057382607221563No Hit
GGGTTAAAGACAGGTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC4970.10531446007797932No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTTATTGGAAAACACCCTCTTTTC4900.10383115782335989No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4900.10383115782335989No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4890.1036192575012714No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT4880.10340735717918291No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4870.10319545685709443No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT4860.10298355653500593No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA4840.10255975589082895No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4810.1019240549245635No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4810.1019240549245635No Hit
GATAGGGGCATTAGGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTC4760.10086455331412103No Hit
ATATTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACC4740.10044075266994405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)