FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471920
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA33910.7185539922020682No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28830.6109086285811155No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC25590.5422529242244448No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC19590.4151127309713511No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG17530.3714612646211222No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACATACT14440.3059840650957789No Hit
CTACTGTACCTGTCTTTAACCCTTATTGGAAAACACCCTCTTTTCCTAAT13460.28521783353110697No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA11970.25364468553992203No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11940.25300898457365656No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA11220.2377521613832853No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10930.23160705204271909No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9920.21020511951178167No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9620.203848109849127No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT9410.19939820308526868No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT8980.19028648923546362No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8730.18498898118325138No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8480.17969147313103914No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8350.1769367689438888No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC8350.1769367689438888No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT8240.1746058654009154No Hit
TCCTCAGGCCATGCAGTGGAACTCAACAACCTTCCACCAAACTCTGCAAG8190.17354636379047297No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8150.17269876250211902No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG8040.1703678589591456No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC7990.16930835734870317No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7900.16740125444990678No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7870.16676555348364128No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTG7820.16570605187319884No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7810.16549415155111036No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT7800.16528225122902188No Hit
ATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGC7740.16401084929649093No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC7720.16358704865231394No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7640.16189184607560603No Hit
ATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGATA7640.16189184607560603No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA7580.1606204441430751No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7430.15744193931174774No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7390.1565943380233938No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7370.15617053737921682No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTG7360.1559586370571283No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT7260.1538396338362434No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7200.1525682319037125No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC7070.14981352771656212No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6920.14663502288523478No Hit
TCTTGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC6860.14536362095270383No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6840.14493982030852687No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTGCACTCAGGCA6800.1440922190201729No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6800.1440922190201729No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6770.14345651805390744No Hit
GTGTAAACAATACATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6750.14303271740973045No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT6710.1421851161213765No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6620.1402780132225801No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATAAGGGTTAAAGA6560.13900661129004915No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6480.13731140871334124No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6430.1362519071028988No Hit
CCCATTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTT6420.1360400067808103No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6350.13455670452619087No Hit
CCTAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACG6320.13392100355992542No Hit
CCTCATCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAGTTAAT6120.12968299711815562No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA6110.12947109679606714No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6090.12904729615189015No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG6050.1281996948635362No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6030.12777589421935923No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5970.12650449228682828No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5850.1239616884217664No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5840.12374978809967792No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5840.12374978809967792No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5760.12205458552297No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5700.12078318359043906No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT5700.12078318359043906No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGTG5540.11739277843702324No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5470.1159094761824038No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5460.11569757586031532No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCTCATCCCATCATCC5440.11527377521613834No Hit
GATGAGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGG5390.11421427360569587No Hit
GAATACAGGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5340.11315477199525344No Hit
TTGTATGCCTGTATTCAATCTAAGCAGGCTTTTTCTTTCTCGCCAACTTA5330.11294287167316495No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5310.11251907102898798No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5250.11124766909645703No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5220.11061196813019156No Hit
GTTTAAATGTATACCCAAAGACAACAGAAAATTGGTAACAGCGGTAAAAA5210.11040006780810308No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC5160.10934056619766061No Hit
GGCCTATTGATTGGAAAGTATGTCAACGTATTGTGGGTCTTTTGGGTTTT5160.10934056619766061No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5150.10912866587557213No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG5130.10870486523139515No Hit
GGTTAAAGACAGGTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACC5130.10870486523139515No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5110.10828106458721817No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATTCAATCTAAGC5110.10828106458721817No Hit
GTCCCGTGCTGGTGTTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5100.10806916426512969No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT5090.1078572639430412No Hit
GTCTTTAACCCTTATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5090.1078572639430412No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC5080.10764536362095271No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG5080.10764536362095271No Hit
CTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA5070.10743346329886422No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5030.10658586201051025No Hit
CCCAAAGACAACAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG5020.10637396168842177No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5010.10616206136633327No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5000.10595016104424479No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5000.10595016104424479No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG4990.1057382607221563No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4960.10510255975589083No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4930.10446685878962535No Hit
GTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA4910.1040430581454484No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4900.10383115782335989No Hit
TCATCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAGTTAATGA4900.10383115782335989No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAGTTAATGACTCTA4880.10340735717918291No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATGTATTGTTTACAC4740.10044075266994405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)