FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488562
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC15630.31991845456666707No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9900.20263548945681406No Hit
CTTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCCGTTTAGCTTG9210.1885124098886119No Hit
GTATCTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACCACAGTGTCA7800.15965220381445958No Hit
GTATAGATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT7550.15453514599989357No Hit
GCCTAAGTCATCCCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTG7530.15412578137472827No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7350.15044149974824075No Hit
ATTAATAGGTGTCAACAATTTGTGGGTCCTCTCACTGTAAATGAAAAGAG7320.1498274528104928No Hit
TTATTAATAGGTGTCAACAATTTGTGGGTCCTCTCACTGTAAATGAAAAG6830.13979801949394344No Hit
GTCCCGTGCTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC6630.13570437324229065No Hit
CCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGAATATGATAAAA6580.13468096167937743No Hit
GTACACAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT6400.13099668005288992No Hit
GTGCTGGCCTGGGCACAGGGTAAGCGTGGGATCCCCCAGGGTTAACGAGT6310.12915453923964615No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6280.1285404923018982No Hit
CTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTTG6250.1279264453641503No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTCATGACTTCTTTCCTTCCGTCAG6230.12751708073898502No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5890.12055788211117525No Hit
TCTTATTGGTTCTTCTGGATTATCAAGGTATGTTGCCCGTTTGTCCTCTA5690.11646423585952242No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT5550.11359868348336548No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5400.11052844879462587No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT5220.10684416716813833No Hit
ATTGATAGGTGTCAACAATTTGTGGGTCCTCTCACTGTAAATGAAAAGAG5170.10582075560522514No Hit
CCGACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATT5020.10275052091648554No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTCTCTAGGGGGAGCACCCGTG4970.10172710935357232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)