FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306893
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGCTGAGGTCAGGTAGCCTAGGAATGCAGTCAAATGAAACATGGC11920.38840898945234986No Hit
GTGCTGGTGTAGTCTTTGCTCTCCAGCTTTGAGACTGTCAAAGAATGTCA11180.3642963508454086No Hit
GTGCTGGGATGGCACATGATGGAGCAGTGTCAGGTAGGAGAGAGGCAGGG10050.32747569999967413No Hit
GTTTTATATTATGGGCAGGACAATTTATGGGCCGCCCCAGCACTCTGCAG7070.2303734526365867No Hit
ATATAAAACACAGCCCTTATTGACAGCTCGTAAAGCAGGGCCCGGTGTCA6790.22124975154206838No Hit
CATGTAACTATGTGTGTGCTCAGTTTCATCTGCCTGACTTGTTTTGAAAA6190.2016989634823864No Hit
GCTCAGGCCAGCTCATGAAATGTCAGTGTTCTAGCAAAAGCACAGGTTGA5520.17986725014907476No Hit
GTGCTGGGCCTAGACGCAGCGGCCGGGACTCCCTAGCCTCAGGCCACCCC4760.15510291860681083No Hit
GTACAGGGATATTCTGTAATTCTTCTATAATGTGATTCTCAATAGAAAAT4680.1524961468655199No Hit
TCCCTGTACTATACACTCACTTTGGAGCACTTTCTGCTTTGTGTTAATTA4520.14728260338293803No Hit
CTCCAGGAGCACAGAGTCTACAGGGAAGTCAGACTGAAAACGGGCATTTC4310.1404398275620493No Hit
TAGTTACATGTGTGCATGCATGCAACACACACATACACATGTGCACACAT4280.13946228815906522No Hit
GTCTACAGGGAAGTCAGACTGAAAACGGGCATTTCAGTAAAATGTTGTAA4260.13881059522374248No Hit
ATTATAGGCTGACTACCAAAATGAAATGAAATAAAGTGACTCATTTCCTT4170.13587797701479018No Hit
GGTGTAGTCTTTGCTCTCCAGCTTTGAGACTGTCAAAGAATGTCAGGAAT4100.1335970517411606No Hit
CACATACACATGTGCACACATGCATGCACGCGCACACACACACACACACA4060.1322936658705151No Hit
CTCCTGCACTGATCACTGCAGTGTGCTTTGTAGCCATCCCCCATCTCTCC3940.12838350825857872No Hit
GCCTATAATGGAGCTTTTCTTGTTTTTCAAAACAAGTCAGGCAGATGAAA3920.12773181532325598No Hit
GTCAATAAGGGCTGTGTTTTATATTATGGGCAGGACAATTTATGGGCCGC3830.12479919711430368No Hit
GTTCATAGGCTGTACAGGAGGCACAATTGGGGAGGCCACAGGAAACTTAC3780.12316996477599684No Hit
CCCCAGCACTCTGCAGACAGCCATTTGGCCAGGGAGGAAAATGGCCTCAT3720.12121488597002864No Hit
GTTTAGCACCATTCTCCTAGTGCTGTTCTTGTGATAAAGTTATCAAAAGA3630.11828226776107634No Hit
GTCTAAATGTAATGCTTTACAAGGCAGGGAGGAGAACCCATTAGGCCTGC3500.11404626368147856No Hit
GTGTATGTGTGTGTTGCATGCATGCACACATGTAACTATGTGTGTGCTCA3500.11404626368147856No Hit
GTGCTGGGGCGGCCCATAAATTGTCCTGCCCATAATATAAAACACAGCCC3410.11111364547252627No Hit
GTACTATACACTCACTTTGGAGCACTTTCTGCTTTGTGTTAATTAAATGT3380.11013610606954215No Hit
GTCTCAGGTAGTTCTTTATAGCAATGTAGAAGCAGACTAATATACCTGAA3360.10948441313421943No Hit
CCTTTACACCATATACAAAACTCAACTCAATGGATTAAAGACTAAAATGT3330.10850687373123531No Hit
CCTCCCACTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3310.1078551807959126No Hit
GTGCTGGTGCATGTGTCTTAATGGTACAACAATTTATGTTCCTTTGGGTA3300.10752933432825122No Hit
TGACAGGACTGACCACCCCTGGTCACCTGCCCCTTCCTCCAAAACCTCAT3280.10687764139292848No Hit
ACCTGAGACTGGGTAATTTATGAAGAAAATAGGTTTAATTGACACAGTTC3200.10427086965163754No Hit
GTGCAGGAGAATGGACTGGAAGCAATCCATGAGATGGAGCCCTTTGGAGT3110.10133825144268524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)