FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473433
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA32650.6896435187238743No Hit
CCTCAAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA28320.5981839035301721No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATACTCTATGA25130.5308037251311168No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG24250.5122160897106877No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC18660.3941423601650075No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGA17470.3690068077214727No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG17130.3618252213090342No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC17010.359290543751703No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC16040.33880190016327544No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT15820.33415499130816817No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15710.33183153688061456No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATACTCTATGA15310.3233826116895105No Hit
CTAACTCTACCAAATATTTGCCCTTGGATAAGGGTATTAAACCGTATTAT14450.3052174225286366No Hit
ATAAAATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAG13790.29127669596331474No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC13570.28662978710820747No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT13030.27522373810021694No Hit
GTATCTTATATAAGAGAGAATCAACACATAGCGCCTCATTTTGTGGGTCA12840.27121049863444247No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC12310.2600156727562295No Hit
AAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC12180.25726977206912066No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAATCAACACATAGCGCC11850.2502994087864598No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11560.24417393802290926No Hit
GGTCTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAG11560.24417393802290926No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA11460.24206170672513325No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA11230.23720357474024834No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG10750.2270648645109234No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA10700.2260087488620354No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC10680.2255863026024802No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACGACAGAGT10470.2211506168771505No Hit
GTAACCTTAGGATAAAACCTAGCAGGCATAATCAATTTTAACCTTCTTTT10400.2196720549687073No Hit
GGTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT9800.2069986671820511No Hit
ATACAAACCTTGCCAACAAATCCGCCTCCTGCCTCAACCAATCGCCAGTC9540.20150686580783342No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9530.2012956426780558No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT9380.1981272957313918No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCTCCTCACCATACTG8870.18735491611273403No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC8860.1871436929829564No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG8750.18482023855540278No Hit
GTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8550.1805957759598507No Hit
GCCTAATCATCGTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTG8420.17784987527274188No Hit
TCCTCAGGCCATGCAGTGGAACTCTACAACCTTCCACCAAACTCTGCAAG8350.17637131336429865No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCTCCTCACCATACTGCACTCAGGCA8320.17573764397496583No Hit
GAATACAGGTGCAGTTTCCATCCGAAGGTTTTGTACAGCAACAGGAGGGA8240.17404785893674501No Hit
GTTGTAGAGTTCCACTGCATGGCCTGAGGATGAGTGTTTCTCAGAGGTGG8010.16918972695186013No Hit
CCTTTAATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG7960.16813361130297214No Hit
GGCCAAGTGTCCTTGTTGGGATTGAAGTCCCAATCTGGATTTGAGGTGTT7870.16623260313497368No Hit
ATATAAGAGAGAATCAACACATAGCGCCTCATTTTGTGGGTCACCATATT7740.16348670244786484No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGATGGAAACTGCACC7650.1615856942798664No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7610.160740801760756No Hit
AATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT7550.1594734629820904No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAACCCTAGAAGACCCACAAT7460.15757245481409196No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGAC7440.15715000855453676No Hit
GTAACTTTAGGATAAAACCTAGCAGGCATAATCAATTTTAACCTTCTTTT7420.15672756229498155No Hit
CATTTAGACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT7330.15482655412698312No Hit
CTATTTACACACTCTATGGAAGGCGGGTATCTTATATAAGAGAGAATCAA7230.1527143228292071No Hit
CTACACACCTTGCCAGCAAATCCGCCTCCTGCCTCAACCAATCGCCAGTC7070.14933475275276545No Hit
CTCTATGACGGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6920.1461664058061014No Hit
GTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG6870.14511029015721336No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6790.14342050511899257No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC6640.1402521581723285No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG6580.1389848193936629No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA6560.13856237313410769No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTT6540.13813992687455248No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG6470.13666136496610926No Hit
GGTGCAAATTTGGAGGATCCAGCATCTAGGGACCTAGTAGTCAGTTATGT6450.13623891870655405No Hit
ATACTAATGTGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCT6430.13581647244699885No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6430.13581647244699885No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT6360.13433791053855562No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGATGGAAACTGC6300.13307057175988998No Hit
CACCTGGCCAGACGCCAACAAGGTAGGGGCTGGAGCATTCGGGCTGGGGT6270.1324369023705572No Hit
CCCTAGAAGACCCACAATTCGTTGACAGACTTTCCAATCAATAGGCCTGT6250.132014456111002No Hit
AACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC6170.13032467107278115No Hit
GTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC6130.12947977855367074No Hit
CTCTCCAACCTTCCACCAAACTCTGCAAGATCCCAGAGTGAGAGGCCTTT6050.1277899935154499No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6040.1275787703856723No Hit
GTGTGAAAGGGGCAGCAAACCCTAGAAGACCCACAATTCGTTGACAGACT5980.1263114316070067No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTACGTTGGCGA5930.12525531595811867No Hit
CATCCAGCGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTGAGTGAT5890.12441042343900827No Hit
GAATACATGCATATAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA5860.12377675404967546No Hit
CCTAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACG5820.12293186153056505No Hit
ATTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5740.12124207649234421No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATTCAATCCAAGC5610.11849617580523537No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGAGC5600.11828495267545779No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCAACCAATCGCCAGTC5570.11765128328612495No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5560.11744006015634736No Hit
CATTTAAACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT5550.11722883702656975No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGGGCAGCA5520.11659516763723694No Hit
CCATTAATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5510.11638394450745934No Hit
CTCCAAAAGACACCAGATACTCTATGACGGTTTCTCTTCCAAAAGTGAGA5510.11638394450745934No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5490.11596149824790415No Hit
CATGTATTCAATCCAAGCAGGCTTTCACTTTCTCGCCAACGTACAAGGCC5340.1127931513012401No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGGTTAAAATTGATTA5290.11173703565235207No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC5280.11152581252257447No Hit
ACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACT5250.11089214313324165No Hit
CCTTCATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTC5230.11046969687368646No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5200.10983602748435364No Hit
GGGTTATTCCCTACATTTCATGGGTTATGTCATTGGATGTTGGGGATCAT5170.10920235809502084No Hit
ATATGATCTTGTGGCAATGATCCCCAACATCCAATGACATAACCCATGAA5160.10899113496524322No Hit
GTATCCATTTAAATACTAACAAAACAAAAAGATGGGGTTATTCCCTACAT5150.10877991183546563No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT5110.10793501931635521No Hit
TTTAAATACTAACAAAACAAAAAGATGGGGTTATTCCCTACATTTCATGG5110.10793501931635521No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTTTGTGCCAAGTGTTTGCTG5100.10772379618657763No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA5100.10772379618657763No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5090.10751257305680001No Hit
TTAGTTCCCCCCAGCAAAGAATGGCTTGCCTGAGTGCAGTATGGTGAGGA5070.1070901267972448No Hit
GCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATACTCTATGAC5020.1060340111483568No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5000.10561156488880158No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4940.10434422611013597No Hit
TCATTAATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG4880.10307688733147034No Hit
CTGCCTCTTCGTCTAACAACAGTAGTCTCCGGAAGTGTTGATAAGATAGG4870.10286566420169274No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4850.10244321794213754No Hit
CTCTATGGAAGGCGGGTATTTTATATAAGAGAGAATCAACACATAGCGCC4820.10180954855280473No Hit
TAACCACATTGTGTGAAAGGGGCAGCAAACCCTAGAAGACCCACAATTCG4780.10096465603369432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)