FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473433
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA32740.6915445268918727No Hit
CCTCAAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA29220.6171939852101564No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATACTCTATGA23280.4917274461222602No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG19370.4091392023792173No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGA15870.3352111069570562No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14750.3115541164219647No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC14340.30289396810108293No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATACTCTATGA14260.3012041830628621No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC14160.2990919517650861No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT13950.2946562660397564No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13350.2819828782531002No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC13200.2788145313064362No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12400.26191668092422793No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC12240.2585371108477863No Hit
ATAAAATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAG12020.25389020199267903No Hit
CTAACTCTACCAAATATTTGCCCTTGGATAAGGGTATTAAACCGTATTAT12020.25389020199267903No Hit
GTATCTTATATAAGAGAGAATCAACACATAGCGCCTCATTTTGTGGGTCA11120.23488012031269473No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAATCAACACATAGCGCC10830.22875464954914423No Hit
AAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC10820.22854342641936665No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT10700.2260087488620354No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA10680.2255863026024802No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC10620.22431896382381455No Hit
GGTCTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAG10140.2141802535944896No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA10110.2135465842051568No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9560.20192931206738865No Hit
GTAACCTTAGGATAAAACCTAGCAGGCATAATCAATTTTAACCTTCTTTT8920.18841103176162202No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACGACAGAGT8830.1865100235936236No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8810.1860875773340684No Hit
GGTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8600.1816518916087387No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8490.17932843718118507No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCTCCTCACCATACTGCACTCAGGCA8480.1791172140514075No Hit
ATACAAACCTTGCCAACAAATCCGCCTCCTGCCTCAACCAATCGCCAGTC8450.17848354466207467No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT8300.17531519771541063No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCTCCTCACCATACTG8070.17045706573052577No Hit
GCCTAATCATCGTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTG7960.16813361130297214No Hit
GTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7680.16221936366919923No Hit
TCCTCAGGCCATGCAGTGGAACTCTACAACCTTCCACCAAACTCTGCAAG7660.16179691740964403No Hit
GGCCAAGTGTCCTTGTTGGGATTGAAGTCCCAATCTGGATTTGAGGTGTT7460.15757245481409196No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7360.15546022351631594No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7330.15482655412698312No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGATGGAAACTGCACC7270.1535592153483175No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC7230.1527143228292071No Hit
CATTTAGACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT7200.15208065343987429No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7190.15186943031009667No Hit
CTCTATGACGGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7160.15123576092076388No Hit
GAATACAGGTGCAGTTTCCATCCGAAGGTTTTGTACAGCAACAGGAGGGA6990.1476449677145446No Hit
CTGCCTCTTCGTCTAACAACAGTAGTCTCCGGAAGTGTTGATAAGATAGG6830.14426539763810295No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG6830.14426539763810295No Hit
GTTGTAGAGTTCCACTGCATGGCCTGAGGATGAGTGTTTCTCAGAGGTGG6820.14405417450832536No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC6820.14405417450832536No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAACCCTAGAAGACCCACAAT6770.14299805885943734No Hit
ATATAAGAGAGAATCAACACATAGCGCCTCATTTTGTGGGTCACCATATT6760.14278683572965972No Hit
CTACACACCTTGCCAGCAAATCCGCCTCCTGCCTCAACCAATCGCCAGTC6610.13961848878299568No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGAC6580.1389848193936629No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT6510.13750625748521966No Hit
GTAACTTTAGGATAAAACCTAGCAGGCATAATCAATTTTAACCTTCTTTT6500.13729503435544205No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG6490.13708381122566446No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC6430.13581647244699885No Hit
CTATTTACACACTCTATGGAAGGCGGGTATCTTATATAAGAGAGAATCAA6430.13581647244699885No Hit
CCCTAGAAGACCCACAATTCGTTGACAGACTTTCCAATCAATAGGCCTGT6090.12863488603456033No Hit
CTCTCCAACCTTCCACCAAACTCTGCAAGATCCCAGAGTGAGAGGCCTTT6000.1267338778665619No Hit
GTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5940.12546653908789626No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5940.12546653908789626No Hit
CCTTTAATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5910.12483286969856347No Hit
GGTGCAAATTTGGAGGATCCAGCATCTAGGGACCTAGTAGTCAGTTATGT5790.12229819214123222No Hit
GTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC5780.12208696901145463No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5700.1203971839732338No Hit
CACCTGGCCAGACGCCAACAAGGTAGGGGCTGGAGCATTCGGGCTGGGGT5670.119763514583901No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTACGTTGGCGA5580.11786250641590257No Hit
TCTCCCGTCCCCTTCTCTGCCTGCCGTTCCGACCGACCACGGGGCGCACC5560.11744006015634736No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTT5540.11701761389679216No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5540.11701761389679216No Hit
CCTAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACG5530.11680639076701455No Hit
GTGTGAAAGGGGCAGCAAACCCTAGAAGACCCACAATTCGTTGACAGACT5510.11638394450745934No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGATGGAAACTGC5510.11638394450745934No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA5480.11575027511812654No Hit
ATACTAATGTGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCT5470.11553905198834893No Hit
AATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT5470.11553905198834893No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCAACCAATCGCCAGTC5380.11363804382035049No Hit
CTCCAAAAGACACCAGATACTCTATGACGGTTTCTCTTCCAAAAGTGAGA5300.11194825878212969No Hit
CATTTAAACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT5290.11173703565235207No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGGGCAGCA5270.11131458939279686No Hit
AACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC5240.11068092000346406No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5180.10941358122479844No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATTCAATCCAAGC5090.10751257305680001No Hit
ATTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5090.10751257305680001No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTTTGTGCCAAGTGTTTGCTG5040.106456457407912No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGGTTAAAATTGATTA4960.10476667236969117No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4930.10413300298035837No Hit
CCTTCATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTC4930.10413300298035837No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA4840.10223199481235995No Hit
GCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATACTCTATGAC4810.10159832542302712No Hit
GGGTTATTCCCTACATTTCATGGGTTATGTCATTGGATGTTGGGGATCAT4750.10033098664436149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)