FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452830
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA22920.5061502108959213No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21010.46397102665459444No Hit
CCTACCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20240.4469668529028554No Hit
GAAAAGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG14240.31446679769449903No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12960.2862001192500497No Hit
TCTGAGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTACTCG11780.26014177505907293No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11670.2577126073802531No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA11600.25616677340282223No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG11240.24821677009032087No Hit
CATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG10540.23275843031601265No Hit
CCTCCCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9870.2179625908177462No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9140.20184175076739616No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8620.19035841264933862No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT8590.18969591237329683No Hit
CTCCTACCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG8340.1841750767396153No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA8200.18108340878475365No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7980.17622507342711394No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAA7970.17600424000176668No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7510.1658459024357927No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7490.16540423558509815No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7460.16474173530905636No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7370.16275423448093101No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAGCAGTCCCCCATG7310.16142923392884748No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7270.16054590022745843No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7260.16032506680211117No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7260.16032506680211117No Hit
ATATTAACGTTGACATAGCTGACCACTAATTCCCTGGATGCTGGATCTTC7180.15855839939933308No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7030.15524589801912417No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7000.15458339774308238No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA6930.15303756376565159No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6800.1501667292361372No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG6750.1490625621094009No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6650.14685422785592828No Hit
ATTTTAGTCTCCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTA6560.14486672702780293No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGCG6550.14464589360245567No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAAGTAGAGGACAAACGGGCAAC6410.141554225647594No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG6360.14045005852085773No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6360.14045005852085773No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6360.14045005852085773No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6300.13912505796877414No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6290.13890422454342688No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT6260.13824172426738512No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6160.13603339001391251No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6140.135591723163218No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAACTCCCCCACTTT6130.13537088973787073No Hit
GAAAGGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG6050.13360422233509264No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6040.13338338890974538No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6040.13338338890974538No Hit
AGTCTAGACTCTGTGGTATTATGAGGATTTTTGTCAACGAGAAAAACCCC5990.13227922178300908No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC5940.13117505465627277No Hit
CATCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG5850.12918755382814742No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCCGC5780.1276417198507166No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5780.1276417198507166No Hit
GCACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5750.12697921957467484No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCGCTAGTAAACTGAG5730.12653755272398032No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCATATAGACCACCAAATGCC5730.12653755272398032No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5680.12543338559724398No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5680.12543338559724398No Hit
GTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCGTTA5650.12477088532120223No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5650.12477088532120223No Hit
CCCCAGCAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA5600.12366671819446591No Hit
ATGCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA5480.12101671709029878No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTC5420.11969171653821523No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGAGGA5400.1192500496875207No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCGTCTGCGAC5390.11902921626217343No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5350.1181458825607844No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5330.11770421571008988No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA5290.11682088200870083No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5270.11637921515800632No Hit
TAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5270.11637921515800632No Hit
GAACAAATGGCGCTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5220.11527504803127No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5210.11505421460592274No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5200.11483338118057548No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5140.11350838062849193No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5140.11350838062849193No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5130.11328754720314467No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC5120.11306671377779741No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5080.11218338007640835No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5010.11063754609897754No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4920.10865004527085219No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4910.10842921184550493No Hit
CTGCTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC4900.10820837842015767No Hit
TCTATCAACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAAGGAGTTT4890.1079875449948104No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC4890.1079875449948104No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4860.10732504471876864No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4830.10666254444272685No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4770.1053375438906433No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4770.1053375438906433No Hit
GACTCAAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACTAAATAT4760.10511671046529603No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG4760.10511671046529603No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCGTCTGCGACGCGGCGA4730.10445421018925424No Hit
GTTTACTAGCGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4700.10379170991321246No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4670.10312920963717069No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4660.10290837621182343No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4650.10268754278647617No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4650.10268754278647617No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4630.10224587593578163No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4600.10158337565973985No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT4600.10158337565973985No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4570.10092087538369808No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4560.10070004195835082No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCG4550.10047920853300356No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA4540.1002583751076563No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4540.1002583751076563No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4540.1002583751076563No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4540.1002583751076563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)