FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452830
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA20790.4591126912969547No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20170.4454210189254245No Hit
CCTACCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17710.39109599628999847No Hit
GAAAAGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG12210.26963761234900513No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA11850.2616876090365038No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11720.2588167745069894No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG10970.24225426760594482No Hit
TCTGAGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTACTCG10940.24159176732990306No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT10850.2396042665017777No Hit
CATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG10830.2391625996510832No Hit
CCTCCCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9180.20272508446878518No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8900.1965417485590619No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8740.19300841375350572No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8630.19057924607468585No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC8270.18262924276218448No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA8210.1813042422101009No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT8200.18108340878475365No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7810.1724709051962105No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7600.16783340326391802No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7570.16717090298787624No Hit
CTCCTACCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7470.16496256873440363No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAA7250.16010423337676388No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7110.15701256542190226No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6930.15303756376565159No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAGCAGTCCCCCATG6870.151712563213568No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6760.14928339553474812No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA6660.1470750612812755No Hit
ATTTTAGTCTCCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTA6510.14376255990106662No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG6440.1422167259236358No Hit
GAACAAATGGCGCTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC6410.141554225647594No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6370.14067089194620497No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6360.14045005852085773No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG6350.14022922509551047No Hit
CATCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG6330.13978755824481595No Hit
ATATTAACGTTGACATAGCTGACCACTAATTCCCTGGATGCTGGATCTTC6300.13912505796877414No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6230.13757922399134334No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6220.13735839056599608No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGCG6210.13713755714064882No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGAGGA6210.13713755714064882No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6160.13603339001391251No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6150.13581255658856525No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6070.13404588918578714No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6040.13338338890974538No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCCGC6000.13250005520835634No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAAGTAGAGGACAAACGGGCAAC5970.13183755493231455No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5880.1298500541041892No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5870.12962922067884194No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT5840.12896672040280016No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5820.12852505355210564No Hit
GAAAGGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG5820.12852505355210564No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5810.12830422012675838No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5750.12697921957467484No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5740.12675838614932758No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5730.12653755272398032No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5710.12609588587328577No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCATATAGACCACCAAATGCC5610.12388755161981317No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAACTCCCCCACTTT5610.12388755161981317No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5570.12300421791842414No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5460.12057505023960427No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5450.12035421681425701No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC5250.1159375483073118No Hit
AGTCTAGACTCTGTGGTATTATGAGGATTTTTGTCAACGAGAAAAACCCC5230.11549588145661728No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCGCTAGTAAACTGAG5220.11527504803127No Hit
GCACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5210.11505421460592274No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC5140.11350838062849193No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5120.11306671377779741No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5100.11262504692710287No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCGTCTGCGAC5060.11174171322571386No Hit
CCCCAGCAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA4990.11019587924828302No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4980.10997504582293577No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4940.10909171212154672No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCGTCTGCGACGCGGCGA4930.10887087869619946No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4870.1075458781441159No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4850.10710421129342138No Hit
GAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTA4800.10600004416668506No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA4760.10511671046529603No Hit
CCTCTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGT4740.1046750436146015No Hit
GTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCGTTA4720.10423337676390698No Hit
TAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4700.10379170991321246No Hit
GTTTACTAGCGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4660.10290837621182343No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4650.10268754278647617No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4640.10246670936112891No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4620.10202504251043437No Hit
GACTCAAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACTAAATAT4580.10114170880904534No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4570.10092087538369808No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC4550.10047920853300356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)