FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235310
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16360.6952530704177469No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12930.5494879095661043No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12400.5269644299009817No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA9100.38672389613701075No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT8400.35697590412647145No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6420.27283158386808887No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6370.2707067272959075No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5950.25285793208958396No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5650.24010879265649568No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5560.2362840508265692No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5550.23585907951213292No Hit
GAGCCTGAGGGCTCCACCCCAACAGTCCCCCATGGGGAGGGGTGAACCCT5320.2260847392800986No Hit
CTCAAGCTCAGGGCCTACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCC5180.22013514087799074No Hit
GTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTT5170.21971016956355446No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5100.21673537036250054No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5030.2137605711614466No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5020.21333559984701034No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5000.21248565721813778No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4960.21078577196039266No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4930.20951085801708386No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4850.20611108750159365No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4770.20271131698610342No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTG4700.19973651778504953No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4640.19718668989843186No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4640.19718668989843186No Hit
CTTTGGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA4570.19421189069737793No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT4550.19336194806850537No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4390.18656240703752497No Hit
CCTCTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGT4320.18358760783647102No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4270.18146275126428965No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4250.18061280863541712No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4110.17466321023330925No Hit
CATTCTGCGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGG4100.174238238918873No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTCCGTAAACAGTATC4060.17253835366112788No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4000.1699885257745102No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT3970.1687136118312014No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG3940.16743869788789256No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA3920.16658875525902No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3900.16573881263014745No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3870.16446389868683864No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3760.1597892142280396No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT3720.1580893289702945No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3700.15723938634142196No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC3640.15468955845480428No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3640.15468955845480428No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC3640.15468955845480428No Hit
GTTCAGAAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA3630.154264587140368No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA3510.1491649313671327No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC3490.14831498873826016No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3480.14789001742382388No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3470.1474650461093876No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA3460.14704007479495135No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3440.1461901321660788No Hit
TCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGG3430.1457651608516425No Hit
CCTGTAAACAGGCCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3400.1444902469083337No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC3400.1444902469083337No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3390.14406527559389742No Hit
CCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCG3390.14406527559389742No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT3350.1423653903361523No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3320.14109047639284347No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3300.14024053376397094No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT3300.14024053376397094No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT3260.13854064850622583No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT3220.1368407632484807No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3200.13599082061960818No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3170.13471590667629935No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGGGC3150.13386596404742682No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG3140.13344099273299054No Hit
GTTTTGTACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTT3100.13174110747524542No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT3080.13089116484637286No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3080.13089116484637286No Hit
CCGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC3070.1304661935319366No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT3060.1300412222175003No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG3060.1300412222175003No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA3040.12919127958862778No Hit
ATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCG3010.12791636564531894No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA3000.12749139433088266No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC2980.12664145170201013No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC2980.12664145170201013No Hit
ACAAGTTGGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGAC2970.12621648038757385No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2910.12366665250095618No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG2870.1219667672432111No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA2870.1219667672432111No Hit
GGGTAAAGGTTCAGATACTGTTTACGGAGAAAGGCCTTGTAAGTTGGCGA2830.12026688198546599No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG2820.11984191067102971No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA2820.11984191067102971No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG2800.11899196804215716No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC2800.11899196804215716No Hit
GGTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGT2780.1181420254132846No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA2750.11686711146997578No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA2740.1164421401555395No Hit
GCTTTGGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG2740.1164421401555395No Hit
CTGTTTACGGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT2740.1164421401555395No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG2720.11559219752666695No Hit
ATCCCGCGGACGACCCCTCCCGGGGCCGCTTGGGGCTCTACCGCCCGCTT2690.11431728358335812No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG2690.11431728358335812No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT2670.11346734095448559No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC2670.11346734095448559No Hit
ATTGTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA2660.1130423696400493No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT2610.11091751306786793No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC2600.11049254175343165No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG2600.11049254175343165No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACGG2590.11006757043899537No Hit
GGATTAAAGCAAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC2580.10964259912455909No Hit
CCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG2560.10879265649568655No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG2550.10836768518125027No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA2540.10794271386681399No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT2530.10751774255237771No Hit
GAGCTTGAGGGCTCCACCCCAACAGTCCCCCATGGGGAGGGGTGAACCCT2520.10709277123794143No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG2510.10666779992350517No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA2500.10624282860906889No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA2480.10539288598019633No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA2480.10539288598019633No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG2470.10496791466576005No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA2440.10369300072245123No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC2420.10284305809357867No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT2410.1024180867791424No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG2410.1024180867791424No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA2410.1024180867791424No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG2390.10156814415026987No Hit
CTCAGGCTCAGGGCCTACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCC2380.10114317283583359No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA2370.1007182015213973No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA2360.10029323020696101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)