FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514635
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA29400.5712786732344283No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT24070.467710124651452No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT20200.3925111972563079No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG18720.36375295112069717No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC17420.3384923295150932No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT15680.30468195905836176No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC15130.29399477299445237No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT14800.28758246135610677No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC14610.2838905243522108No Hit
GTATAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC14150.2749521505533048No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC13820.26853983891495914No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12700.24677684183936188No Hit
TCCTCAGGCCGTGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG11420.22190484518153641No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG11350.2205446578643116No Hit
CCCTTAACTTCATGGGATATGTAATCGGAAGTTGGGGTACCTTACCACAG11230.21821290817764047No Hit
TTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTC11210.21782428322986191No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10590.20577690984872773No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10210.19839303584093582No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG10140.197032848523711No Hit
GTCCCGTGCTGGTAGTTGATGTTCCGTTTCCTGGAAGTAGAGGACAAACG9850.1913977867809224No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9780.19003759946369758No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9740.18926034956814053No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA9730.18906603709425127No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG9670.18790016225091571No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT9640.18731722482924792No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9620.1869285998814694No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT9330.18129353813868082No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9250.17973903834756672No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9230.17935041339978822No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG9080.17643572629144927No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC8870.1723551643397748No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8810.17118928949643925No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG8800.17099497702254995No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT8680.16866322733587882No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8460.16438835291031506No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT8380.16283385311920098No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC8310.16147366580197617No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATTATGCCTGCTAGG8100.15739310385030167No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCGATTACATATCCCATGAA8070.1568101664286339No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATTATG7890.1533125418986272No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7850.15253529200307014No Hit
TGTTTATACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT7820.15195235458140235No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCTC7810.1517580421075131No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7780.1511751046858453No Hit
ACACGTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA7690.14942629242084196No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7690.14942629242084196No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG7650.1486490425252849No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7510.14592866789083525No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7500.145734355416946No Hit
GCACTAAGGCAAGCTATCCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7490.14554004294305672No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG7460.14495710552138893No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC7350.14281966830860707No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7340.1426253558347178No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7340.1426253558347178No Hit
TACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGC7280.14145948099138222No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC7260.1410708560436037No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7220.14029360614804667No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7080.13757323151359702No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6950.13504716935303662No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6890.13388129450970104No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCG6870.13349266956192254No Hit
GGGTAAAGGTTCAGATATTGTTTATACAGAAAGGCCTTGTAAGTTGGCGA6860.13329835708803325No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC6810.13232679471858697No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCGTTAAC6760.13135523234914065No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6650.12921779513635878No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6600.12824623276691247No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG6490.1261087955541306No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT6480.12591448308024134No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6470.12572017060635207No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6460.1255258581324628No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG6430.12494292071079505No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6430.12494292071079505No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG6430.12494292071079505No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6420.12474860823690577No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6420.12474860823690577No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6400.12435998328912724No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6370.12377704586745947No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT6360.12358273339357019No Hit
TTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATA6360.12358273339357019No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6340.12319410844579169No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC6310.12261117102412389No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6260.12163960865467759No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6260.12163960865467759No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6220.12086235875912053No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG6160.11969648391578498No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA6130.1191135464941172No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6120.11891923402022793No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6080.11814198412467088No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6060.11775335917689235No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC6030.11717042175522457No Hit
ATACAGCACTAAGGCAAGCTATCCTGTGTTGGGGTGAGTTGATGAATCTG5980.11619885938577827No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5960.11581023443799975No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5940.11542160949022122No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGAGAGG5900.1146443595946642No Hit
CTCCTACCTGGTGTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC5860.11386710969910714No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCC5830.11328417227743937No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT5830.11328417227743937No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5790.11250692238188231No Hit
GTACAAAGACCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTA5770.11211829743410379No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5760.11192398496021451No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5750.11172967248632526No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5710.11095242259076821No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5700.11075811011687896No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5690.11056379764298968No Hit
ATCCCAGAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGA5670.11017517269521118No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5670.11017517269521118No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5620.10920361032576485No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5610.1090092978518756No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5590.10862067290409708No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTCC5570.10823204795631855No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG5570.10823204795631855No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5570.10823204795631855No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACCTTT5450.10590029826964742No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5440.10570598579575817No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT5440.10570598579575817No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5430.10551167332186889No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAACGGAACATCA5430.10551167332186889No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5420.10531736084797964No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5420.10531736084797964No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5410.10512304837409037No Hit
GTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATAC5370.10434579847853334No Hit
TCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTG5350.10395717353075482No Hit
AGATAGGGGCATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACT5340.10376286105686554No Hit
TCACCATACAGCACTAAGGCAAGCTATCCTGTGTTGGGGTGAGTTGATGA5330.10356854858297629No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCGTTTCCTGGAAGTA5330.10356854858297629No Hit
CTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTCCC5300.10298561116130851No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5280.10259698621352999No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCC5260.10220836126575146No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGA5260.10220836126575146No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC5240.10181973631797293No Hit
GGAACATATTGTACACAAAATCAAACAATGCTTTCGGAAACTTCCTGTAA5220.10143111137019441No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT5220.10143111137019441No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5210.10123679889630516No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5160.10026523652685886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)