FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269612
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCATGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA15190.5634022224530065No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA15160.5622895123362461No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAGAGTCCAA11130.4128154533181016No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT10700.3968666083112028No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA10640.394641188077682No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10420.38648131388810586No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT10070.37349969585923476No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9600.35606723736332213No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT8830.32750767769980565No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8060.2989481180362892No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7460.2766939157010816No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCA7190.2666795246502381No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6730.24961796952657891No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6630.24590893580404435No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG6500.24108719196474934No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6390.23700725486996127No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCACAT6110.2266219604468644No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5950.22068750649080904No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5910.21920389300179519No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5770.21401124579024672No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5760.2136403424179933No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGT5580.20696408171743097No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5540.20548046822841715No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCA5530.2051095648561637No Hit
GCCTAGCAGCCATGGAAAGGATGTGTATTTCCGAGAGAGGACAACAGAGT5430.20140053113362907No Hit
AGTCTAGACTCTGTGGCATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5260.19509517380532024No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCA5160.19138614008278565No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA5090.1887898164770114No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC5050.18730620298799758No Hit
ATACTGCGGAACTCCTGGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCA5040.18693529961574412No Hit
GGATATTATCAATAGATGTCAGCATTTCGTGGGCCCACTTACAGTTAATG4990.1850807827544768No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG4880.18100084565968874No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG4820.17877542542616798No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGA4770.17692090856490067No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTTCTCCTCCC4700.17432458495912645No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA4670.17321187484236605No Hit
GTGGCATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCGGTAACACG4650.17247006809785914No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4600.17061555123659183No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC4370.16208477367476223No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4200.15577941634645343No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4150.15392489948518612No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4150.15392489948518612No Hit
GGTTCGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCA4100.1520703826239188No Hit
ATTTACAGGAAGTTTCCGAAAACACTGTTTGATTTTTTGTACAATATGTT4040.14984496239039805No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4030.1494740590181446No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4000.14836134890138422No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC4000.14836134890138422No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG3970.14724863878462383No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCTCACTCTGGG3970.14724863878462383No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3940.14613592866786346No Hit
ATTTATAGGAAGTTTCCGAAAACACTGTTTGATTTTTTGTACAATATGTT3930.14576502529560997No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3930.14576502529560997No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGG3920.14539412192335655No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC3850.1427977983175823No Hit
CATACTGCGGAACTCCTGGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC3840.14242689494532884No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT3810.14131418482856847No Hit
CGTCAGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCGTTTGGGACTC3760.13945966796730117No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC3670.13612153761702No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT3650.1353797308725131No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3630.13463792412800615No Hit
TTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCT3600.13352521401124579No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3500.12981618028871117No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG3490.12944527691645774No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3470.1287034701719508No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG3450.1279616634274439No Hit
GTGGATACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG3450.1279616634274439No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3430.12721985668293698No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3370.12499443644941621No Hit
ATCCGAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT3360.12462353307716274No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA3300.12239811284364198No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3230.11980178923786775No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCATTAAC3220.1194308858656143No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTAT3220.1194308858656143No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC3200.11868907912110738No Hit
CCCAGTTCTCCTCCCCCAACTCCTCCCAGTCTGTAAATACACAGTCTTTG3190.11831817574885391No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT3180.11794727237660046No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3180.11794727237660046No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3160.11720546563209351No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGC3150.11683456225984007No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG3140.1164636588875866No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA3120.11572185214307967No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3110.11535094877082623No Hit
GGGTAAAGGTTCAGATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA3100.11498004539857276No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA3080.11423823865406584No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG3080.11423823865406584No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT3070.11386733528181239No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC3050.11312552853730547No Hit
GGAACATATTGTACAAAAAATCAAACAGTGTTTTCGGAAACTTCCTATAA3050.11312552853730547No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3030.11238372179279854No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC3010.11164191504829163No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2990.1109001083037847No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT2940.1090455914425174No Hit
ATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT2910.10793288132575701No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG2910.10793288132575701No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA2890.10719107458125009No Hit
ATAATATCCTCTTTCAAATGAATATGTGGAAAAGAGGGAGTTTGCCATTC2870.10644926783674316No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2870.10644926783674316No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA2860.10607836446448972No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG2840.10533655771998279No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA2840.10533655771998279No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT2830.10496565434772932No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC2830.10496565434772932No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2830.10496565434772932No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACA2830.10496565434772932No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACGACTATT2790.10348204085871548No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC2790.10348204085871548No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG2780.10311113748646202No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT2780.10311113748646202No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG2750.10199842736970165No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG2750.10199842736970165No Hit
CCTCAAGGTCGGTCGTTGACATTGTTGAGAGTCCAAGAGTCCTCTTATGT2750.10199842736970165No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT2750.10199842736970165No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2730.10125662062519472No Hit
TGGAAACCACCGTGAACGCCCGCCAGGTCTTGCCTAAGGTCTTACATAAG2720.10088571725294125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)