FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269612
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCATGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA14030.5203774312716052No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA13020.48291619067400554No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10880.403542869011765No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT9940.36867795201993975No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT9440.3501327834072668No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAGAGTCCAA9150.33937658561191636No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8860.328620387816566No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8710.3230568372327641No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8600.3189769001379761No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT8050.29857721466403575No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7850.2911591472189665No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCA7150.26519591116122426No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG6820.2529560998768601No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6690.2481343560375651No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6470.23997448184798895No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5960.22105840986306247No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5880.2180911828850348No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCACAT5740.21289853567348632No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5730.2125276323012329No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5550.20585137160067057No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCA5530.2051095648561637No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5380.19954601427236177No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCA5180.19212794682729256No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGT5110.1895316232215183No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5070.1880480097325045No Hit
GGATATTATCAATAGATGTCAGCATTTCGTGGGCCCACTTACAGTTAATG5030.18656439624349064No Hit
ATACTGCGGAACTCCTGGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCA4950.18359716926546296No Hit
GCCTAGCAGCCATGGAAAGGATGTGTATTTCCGAGAGAGGACAACAGAGT4950.18359716926546296No Hit
AGTCTAGACTCTGTGGCATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4890.1813717490319422No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG4860.1802590389151818No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC4810.1784045220539145No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA4790.17766271530940758No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG4750.17617910182039376No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4700.17432458495912645No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4560.169131937747578No Hit
CGTCAGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCGTTTGGGACTC4530.1680192276308176No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGA4330.1606011601857484No Hit
GTGGCATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCGGTAACACG4320.16023025681349495No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4270.15837573995222765No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC4250.15763393320772073No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC4240.15726302983546725No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA4170.15466670622969306No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4130.1531830927406792No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4060.15058676913490499No Hit
ATTTACAGGAAGTTTCCGAAAACACTGTTTGATTTTTTGTACAATATGTT4000.14836134890138422No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTTCTCCTCCC3970.14724863878462383No Hit
ATTTATAGGAAGTTTCCGAAAACACTGTTTGATTTTTTGTACAATATGTT3830.14205599157307539No Hit
GTGGATACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG3830.14205599157307539No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG3810.14131418482856847No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3780.14020147471180808No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3730.1383469578505408No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3690.13686334436152692No Hit
GGTTCGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCA3660.13575063424476655No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3580.13278340726673887No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3530.13092889040547157No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3530.13092889040547157No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3440.1275907600551904No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG3440.1275907600551904No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCTCACTCTGGG3370.12499443644941621No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3360.12462353307716274No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3330.12351082296040235No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG3290.12202720947138851No Hit
GGGTAAAGGTTCAGATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA3280.12165630609913505No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG3240.12017269261012122No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3180.11794727237660046No Hit
TTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCT3150.11683456225984007No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC3150.11683456225984007No Hit
ATCCGAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT3140.1164636588875866No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3140.1164636588875866No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC3130.11609275551533314No Hit
CATACTGCGGAACTCCTGGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC3120.11572185214307967No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3090.1146091420263193No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGG3090.1146091420263193No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC3010.11164191504829163No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT2980.11052920493153123No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC2970.11015830155927776No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG2960.10978739818702432No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA2910.10793288132575701No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCATTAAC2880.10682017120899664No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT2870.10644926783674316No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC2860.10607836446448972No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC2860.10607836446448972No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA2860.10607836446448972No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC2830.10496565434772932No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA2830.10496565434772932No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG2830.10496565434772932No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT2810.10422384760322241No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG2800.10385294423096895No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT2790.10348204085871548No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC2780.10311113748646202No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT2770.10274023411420857No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT2760.1023693307419551No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2760.1023693307419551No Hit
TGGAAACCACCGTGAACGCCCGCCAGGTCTTGCCTAAGGTCTTACATAAG2740.10162752399744818No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT2740.10162752399744818No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCA2730.10125662062519472No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2720.10088571725294125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)