FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461194
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC27590.5982298121831594No Hit
ATTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT23210.503258932249769No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21350.462928832552028No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA20050.43474112846220897No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGAG17570.38096766219855416No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC16590.3597184698846906No Hit
GTTGTGAATATGCCCTGAGCCTGAGGGCTCCACCCCAATAGTCCCCCATG15560.33738513510583396No Hit
ACCTCTTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCG15360.3330485652458618No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA14110.3059450036210358No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT13910.30160843376106367No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC12730.27602267158722793No Hit
ACAATAGGTTGGTGAGTGACTGCAGATTTGGGACTGCGAATTTTGGCCAA11590.25130422338538666No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11340.24588351106042142No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG11200.2428479121584409No Hit
CCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG10940.2372103713404771No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10630.23048868805752026No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT10620.23027185956452165No Hit
CTATTGGGGTGGAGCCCTCAGGCTCAGGGCATATTCACAACTGTGCCAGC10600.22983820257852444No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10360.22463431874655787No Hit
CCCCAATAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA10190.22094823436558153No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC10070.21834629244959822No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9600.20815535327866364No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9520.2064207253346748No Hit
CCCTTATCTCCACCTCTAAGGGACACTCACCCTCAGGCCATGCAGTGGAA9310.201867326981704No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT8820.19124273082477222No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT8740.18950810288078335No Hit
GCCTGAGGGTGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT8730.18929127438778473No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC8660.1877734749367945No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA8580.18603884699280562No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCAAACTTACAAGACC8400.1821359341188307No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8150.17671522179386548No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8140.17649839330086686No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA8080.17519742234287525No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8080.17519742234287525No Hit
GTAGTAGGCTATGTCAACGTTAATATGGGCCTAAAATTCAGACAACTATT8050.1745469368638794No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC8000.17346279439888637No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG7970.17281230891989055No Hit
GAATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7910.17151133796189888No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA7830.16977671001791003No Hit
CCTCTAAGGGACACTCACCCTCAGGCCATGCAGTGGAACTCCACCACTTT7630.16544014015793787No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7620.16522331166493928No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG7620.16522331166493928No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7610.16500648317194067No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7580.16435599769294484No Hit
ACTGCAGATTTGGGACTGCGAATTTTGGCCAAGACACTCGGGTGTTCCCC7570.16413916919994623No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7570.16413916919994623No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7390.1602362563259713No Hit
CCCTTACAGTAAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7380.16001942783297268No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA7310.15850162838198242No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7170.1554660294800019No Hit
ATATTAACGTTGACATAGCCTACTACTAATTCCCTGGATGCTTGATCTTG7150.1550323724940047No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7130.1545987155080075No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC7070.15329774455001582No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG6890.1493948316760409No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6820.14787703222505064No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT6750.14635923277406038No Hit
GGAACATATTGTACAAAAACTCAAAATGTGTTTTAGAAAACTTCCTGTAA6630.14375729085807706No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA6610.14332363387207986No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6610.14332363387207986No Hit
CTCCAAAAGACACCAAATATTCAAGAACCGTTTCTCTCCCAAAAGTAAGA6590.14288997688608265No Hit
GGATAGGGGCATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACT6540.14180583442108963No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC6510.14115534894209378No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6480.14050486346309796No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6450.13985437798410214No Hit
CCTTATGAGTCCAAGGAATATTAACATTGAGATTCCCGAGATTGAGATCT6380.13833657853311188No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCCTCTGTGG6350.13768609305411605No Hit
GCCTACTACTAATTCCCTGGATGCTTGATCTTGCAAATTACTTCCCACCC6270.13595146511012718No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG6250.13551780812412997No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6170.13378318018014113No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6150.1333495231941439No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT6120.13269903771514807No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6080.13183172374315366No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6050.1311812382641578No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6020.130530752785162No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6020.130530752785162No Hit
ACCTATTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCG5970.12944661032016896No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGAGTTTTTGT5950.12901295333417176No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5920.1283624678551759No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5900.1279288108691787No Hit
CCCCTATCCTATCAACACTTCCGGAAACTACTGTTGTTAGACGAAGAGGC5890.1277119823761801No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5860.12706149689718427No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5790.125543697446194No Hit
CTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTA5750.12467638347419957No Hit
GTATAAAGAATTTGGAGCCTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5740.12445955498120098No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG5740.12445955498120098No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGATGGAGTTT5690.12337541251620794No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5680.12315858402320932No Hit
GCTTTAATCCTAAATGGCAAACTCCATCTTTTCCTGACATTCATTTGCAG5660.1227249270372121No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5650.12250809854421349No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5610.12164078457221907No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACTT5560.12055664210722603No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5540.12012298512122882No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5540.12012298512122882No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGC5530.1199061566282302No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG5520.11968932813523159No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATGCC5510.119472499642233No Hit
GGATATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5500.11925567114923438No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5490.11903884265623577No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5490.11903884265623577No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGAGTTTTTGTACAATATGTT5410.11730421471224692No Hit
TCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTT5410.11730421471224692No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5390.11687055772624969No Hit
GTCCAAGGAATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5360.11622007224725388No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTACTGCCAACTGGATCCTAC5310.11513592978226082No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5300.11491910128926222No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG5200.11275081635927614No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACGTTTCCT5200.11275081635927614No Hit
GGCTTTTACTTTCTCGCAAACTTACAAGACCTTTCTAAGTAAACAGTATC5190.11253398786627752No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGTCTTGTAAGTTTGCGA5180.11231715937327894No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5180.11231715937327894No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5180.11231715937327894No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGATGGAGT5150.11166667389428311No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5150.11166667389428311No Hit
GTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5150.11166667389428311No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5120.11101618841528728No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5100.11058253142929007No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT5010.10863107499230258No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5000.10841424649930399No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4980.10798058951330676No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATATTAAC4970.10776376102030816No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4960.10754693252730954No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA4940.10711327554131234No Hit
GGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGG4900.1062459615693179No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4900.1062459615693179No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4890.1060291330763193No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA4850.10516181910432486No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC4840.10494499061132626No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG4820.10451133362532905No Hit
CCTGCATATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGAAAC4800.10407767663933182No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC4800.10407767663933182No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA4790.10386084814633322No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4750.10299353417433878No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4750.10299353417433878No Hit
GATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA4740.10277670568134017No Hit
GTGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4740.10277670568134017No Hit
CTCTTATGCAAGACCTTGGGCAAGTTCCGGTGGATGTTCACGGTGGTCTC4710.10212622020234435No Hit
GACAATAGGTTGGTGAGTGACTGCAGATTTGGGACTGCGAATTTTGGCCA4700.10190939170934574No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA4660.10104207773735131No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4640.10060842075135411No Hit
GTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGG4620.10017476376535688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)