FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461194
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC25100.544239517426506No Hit
ATTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT21190.4594595766640503No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA19630.4256343317562674No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA18490.4009158835544261No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGAG17450.3783657202825709No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA15100.327411024427898No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC14520.3148349718339788No Hit
ACCTCTTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCG13980.3031262332120539No Hit
GTTGTGAATATGCCCTGAGCCTGAGGGCTCCACCCCAATAGTCCCCCATG13640.2957540644501013No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT12880.2792750989822071No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC11660.2528220228363769No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11250.24393205462343395No Hit
CCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG10140.21986409190058848No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT9940.21552752204061632No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC9910.21487703656162047No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9780.2120582661526386No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9550.20707121081367058No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9420.2042524404046887No Hit
ACAATAGGTTGGTGAGTGACTGCAGATTTGGGACTGCGAATTTTGGCCAA9420.2042524404046887No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT9350.20273464095369845No Hit
CTATTGGGGTGGAGCCCTCAGGCTCAGGGCATATTCACAACTGTGCCAGC9320.20208415547470263No Hit
CCCCAATAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA9280.20121684150270822No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9220.19991587054471655No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT8500.18430421904881675No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA8440.18300324809082513No Hit
CCCTTATCTCCACCTCTAAGGGACACTCACCCTCAGGCCATGCAGTGGAA8420.1825695911048279No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8160.1769320502868641No Hit
GCCTGAGGGTGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT8070.17498059384987663No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT8020.17389645138488358No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA7940.17216182344089473No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG7880.17086085248290306No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7870.17064402398990447No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC7870.17064402398990447No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7820.16955988152491142No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7770.1684757390599184No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC7750.16804208207392118No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCAAACTTACAAGACC7480.16218771276295876No Hit
GTAGTAGGCTATGTCAACGTTAATATGGGCCTAAAATTCAGACAACTATT7460.16175405577696153No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA7420.1608867418049671No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7420.1608867418049671No Hit
ATATTAACGTTGACATAGCCTACTACTAATTCCCTGGATGCTTGATCTTG7260.1574174859169894No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7130.1545987155080075No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7110.15416505852201026No Hit
GAATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7080.15351457304301444No Hit
CCTCTAAGGGACACTCACCCTCAGGCCATGCAGTGGAACTCCACCACTTT7050.15286408756401862No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7040.15264725907102No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7010.15199677359202418No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6970.15112945962002974No Hit
CTCCAAAAGACACCAAATATTCAAGAACCGTTTCTCTCCCAAAAGTAAGA6820.14787703222505064No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG6800.1474433752390534No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT6650.1441909478440743No Hit
GCCTACTACTAATTCCCTGGATGCTTGATCTTGCAAATTACTTCCCACCC6560.14223949140708683No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6490.14072169195609657No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6460.14007120647710075No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6400.13877023551910908No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6350.13768609305411605No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA6340.13746926456111744No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6320.13703560757512023No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6290.1363851220961244No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCCTCTGTGG6250.13551780812412997No Hit
CCCTTACAGTAAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6240.13530097963113136No Hit
ACTGCAGATTTGGGACTGCGAATTTTGGCCAAGACACTCGGGTGTTCCCC6170.13378318018014113No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6140.13313269470114528No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6070.13161489525015505No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG6060.13139806675715643No Hit
GGATAGGGGCATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACT5960.12922978182717035No Hit
GGAACATATTGTACAAAAACTCAAAATGTGTTTTAGAAAACTTCCTGTAA5960.12922978182717035No Hit
CCTTATGAGTCCAAGGAATATTAACATTGAGATTCCCGAGATTGAGATCT5950.12901295333417176No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5950.12901295333417176No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG5920.1283624678551759No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5920.1283624678551759No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5880.12749515388318147No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5810.1259773544321912No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGAGTTTTTGT5790.125543697446194No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5780.1253268689531954No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5770.1251100404601968No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5770.1251100404601968No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5760.12489321196719819No Hit
CTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTA5670.12294175553021071No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5650.12250809854421349No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5620.12185761306521767No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGAGTTTTTGTACAATATGTT5600.12142395607922045No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5500.11925567114923438No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5480.11882201416323716No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5480.11882201416323716No Hit
GTCCAAGGAATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5420.1175210432052455No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGATGGAGTTT5410.11730421471224692No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5400.1170873862192483No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5400.1170873862192483No Hit
GTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5350.11600324375425526No Hit
GTATAAAGAATTTGGAGCCTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5350.11600324375425526No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5340.11578641526125664No Hit
CCCCTATCCTATCAACACTTCCGGAAACTACTGTTGTTAGACGAAGAGGC5330.11556958676825806No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5320.11535275827525944No Hit
GGATATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5320.11535275827525944No Hit
ACCTATTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCG5310.11513592978226082No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5270.1142686158102664No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5240.11361813033127056No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGC5210.11296764485227474No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACTT5190.11253398786627752No Hit
GAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTA5170.11210033088028032No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA5160.1118835023872817No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTACTGCCAACTGGATCCTAC5140.1114498454012845No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5110.11079935992228866No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATGCC5080.11014887444329284No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5000.10841424649930399No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT4940.10711327554131234No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG4920.10667961855531513No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4910.10646279006231651No Hit
GGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGG4880.10581230458332068No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4870.10559547609032209No Hit
GTGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4850.10516181910432486No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA4830.10472816211832764No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4830.10472816211832764No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGTCTTGTAAGTTTGCGA4800.10407767663933182No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA4760.1032103626673374No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG4690.10169256321634715No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACGTTTCCT4660.10104207773735131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)