FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590883
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA23570.39889453580488865No Hit
GTTACTCTTCCAAACATAAGACAGGAAATGTGAAACCACAATAGTTGTCT20240.34253820130211904No Hit
CTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT19950.3376302922913673No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAATTGTGTGTTCAGG18310.3098752206443577No Hit
ATGTCAATGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCAC18020.30496731163360596No Hit
ATCCCGATTGGGACTTCAACCCCAACAAGGATCAATGGCCAGCGGCACAC15780.26705794548159284No Hit
GAAGACAAACTGTTCTTGAGTTTTTGGTGTCTTTTGGAGTGTGGATTCGC15540.2629962276795914No Hit
GTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGGTAGT15520.26265775119609125No Hit
GTGTAAAGGGGGCGGCAAAGCCCAGGAGCCCCACAATTCGTTGACATACT14940.25284193317458786No Hit
AATCTGGATTGTTTGAATTGGCTCCGAATGCAGGGTCCAACTGGTGACCG14820.2508110742735872No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC14570.24658011822983572No Hit
GGATATTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA14080.23828744438408278No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTATTCCCTTAACTTCAT13710.23202562943933064No Hit
ACACCGCCACTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAGCATTGT13700.23185639119758056No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT13290.22491762328582815No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12920.218655808341076No Hit
GAAGAGTAACTGTTCTTGAGTTTTTGGTGTCTTTTGGAGTGTGGATTCGC12350.20900922856132262No Hit
ATGTAAGACCTTGGGCAAGGCCTGTCGGGCGTTCACGGTGGTCTCCATGC12080.20443979603407106No Hit
AGTCTAGACTCTGTGGTATTGTGTGGATTTTTGTCAACAAGAAAAACCCC11560.195639407463068No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG10920.1848081599910642No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC10890.18430044526581404No Hit
GTTTGTCTTCCAAACATAAGACAGGAAATGTGAAACCACAATAGTTGTCT10660.1804079657055627No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG10620.17973101273856246No Hit
TAGATAAAGGCATCAAACCTTATTATCCTGAACACACAATTAATCATTAC10560.17871558328806209No Hit
GTAGTAAACTATGTCAATGTTAATATGGGCATAAAAATCAGACAACTATT10370.17550005669481097No Hit
CTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT10330.17482310372781076No Hit
GTCTATAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAAAAC10170.1721152918598098No Hit
GTATAGGCCCCTCACTCTGGGATCTAGTAGAGCTTGGTGGAATGTGCTGG10010.16940747999180886No Hit
ATGTTAATGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCAC9880.16720738284905812No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG9650.16331490328880674No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG9390.1589147090033052No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9340.1580685177945549No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAGCATTGTTCACCTCACCATACAG9300.1573915648275547No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTACTAG9220.15603765889355423No Hit
CCTTATGAGTCCAAGGGATATTAACATTGGGATTCCCGAGATTGAGATCT9110.1541760382343036No Hit
GTTTACTACTGCGTCCCTGGAAGCTGGGTCAATCAAATTACCTCCCACCC9070.15349908526730335No Hit
ATCTGTACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG9060.15332984702555327No Hit
CTACAGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8820.14926812922355187No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC8610.14571412614680065No Hit
ATATGTTCCTGTGGTAAGGAACCCCAACTTCCAATTACATAACCCATGAA8590.14537564966330052No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTAATCTCTTTTTTGCCTTCTG8590.14537564966330052No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG8580.14520641142155044No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC8540.14452945845455023No Hit
TTTTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT8410.14232936131179946No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG8380.14182164658654928No Hit
GTGTAAACAATATCTGTACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC8360.14148317010304917No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC8340.14114469361954904No Hit
CATTAACATAGTTTACTACTGCATCCCTGGAAGCTGGGTCAATCAAATTA8320.14080621713604893No Hit
GAACCGAAAGATTCGTCCCCATGCCTTGTCGAGGTTTGGAAGACCAACCT8310.14063697889429885No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT8290.14029850241079875No Hit
TTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGGTCACCAGTTGGACCCT8280.1401292641690487No Hit
CTCCTACCTGGTGTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC8160.13809840526804798No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8120.13742145230104774No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGTGAGAGGACAACAGAGT8080.13674449933404753No Hit
CCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATA7970.13488287867479687No Hit
GTCCAAGGGATATTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGAC7940.1343751639495467No Hit
GAATCTGGCCACCTGGGTGGGAGGTAATTTGATTGACCCAGCTTCCAGGG7620.1289595402135448No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7510.12709791955429417No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG7430.1257440136202937No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAATTGTG7380.1248978224115434No Hit
GTCCTCTCACGGAAATACACCTCCTTTCCATGGCTGCTAGGCTGTGCTGC7330.12405163120279311No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTATTAGACGACGAGTC7310.12371315471929299No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7230.12235924878529253No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCG7210.1220207723017924No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG7190.12168229581829228No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC7100.12015915164254175No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCCTGCATGGTCCCGTG7090.1199899134007917No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGAACCCA7070.11965143691729159No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC6980.11812829274154103No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC6970.117959054499791No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6960.11778981625804094No Hit
GTGGTATTGTGTGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6960.11778981625804094No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG6950.11762057801629087No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6880.11643591032404045No Hit
CCTTTCTCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGG6770.11457428966478983No Hit
GGGTAAAGGTACAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6750.1142358131812897No Hit
CAGGACGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCT6740.11406657493953963No Hit
GTCCCGTGCTTGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6730.11389733669778956No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6700.1133896219725394No Hit
CCCTTAACTTCATGGGTTATGTAATTGGAAGTTGGGGTTCCTTACCACAG6690.11322038373078934No Hit
CTCTGGGATCTAGTAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCATG6690.11322038373078934No Hit
ATCCAGATTGGGACTTCAACCCCAACAAGGATCAATGGCCAGCGGCACAC6640.11237419252203905No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6500.11000485713753823No Hit
GTGATAGGACAACAGAGTTGTCGGTGCCGATAAGTTTTGCTCCAGACCGG6470.10949714241228804No Hit
GTACACCATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6440.10898942768703787No Hit
GCACGGGACCATGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6440.10898942768703787No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6410.1084817129617877No Hit
CTGTGGTATTGTGTGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA6330.10712780702778722No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6280.10628161581903693No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG6270.10611237757728686No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6260.10594313933553683No Hit
TCTGTGGAGTTAATCTCTTTTTTGCCTTCTGACTTCTTTCCTACTATTCG6250.10577390109378676No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGAACCCACTCCTACCTGGTGTGCC6240.1056046628520367No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAGCATTGTTCACCTCACCA6230.10543542461028664No Hit
CCAACCTCTTGTCCTCCGACTTGTCCTGGTTATCGCTTGATGTGTCGGCG6230.10543542461028664No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTTC6180.10458923340153634No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA6140.10391228043453611No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6120.10357380395103599No Hit
CAGGAACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTC6100.10323532746753589No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGGCCTGTCGGGCGTTC6080.10289685098403575No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG6020.1018814215335354No Hit
CAATTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAG5980.10120446856653517No Hit
GATTAATTGTGTGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGC5940.10052751559953493No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5930.10035827735778488No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5920.10018903911603481No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACA5910.10001980087428475No Hit
ATATAAGAGAGAAACTACTCGAAGCGCCTCATTTTATGGGTCACCATATT5910.10001980087428475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)