FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391684
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21590.551209648594275No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTTGTTGACATTGCTGGGAGTCCAA14120.3604946844905587No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12440.317602965655988No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG12120.3094331144494031No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA11130.28415763727903104No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10630.27139224476874213No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT10400.26552016421400926No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC10360.26449893281318615No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG10050.256584389456807No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTATATACCCCATGAA10020.2558184659061897No Hit
ACATCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAAC9890.25249946385351457No Hit
GTACGGGACCTTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG9730.24841453825022208No Hit
GTTTTGATGACCAACCTCCCATGATGTAGCTCTTGTTCCCAAGAATATGG9360.2389681477926083No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT9100.2323301436872581No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8830.2254368317317021No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC8770.22390498463046743No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8410.21471390202305937No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGGAAACTTCCTGTCA8180.2088418214683265No Hit
ACATCAAACCTCGCAAAGGCATGGGGACGAACCTTTCTGTTCCCAACCCT8140.20782059006750336No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT7470.19071496410371627No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG7410.18918311700248158No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA7370.18816188560165847No Hit
GTTTGATGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGC7330.18714065420083537No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC7120.18177918934651402No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT6780.17309872243951757No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTGTT6750.17233279888890024No Hit
ATTATATACCCCATGAAATTTAGGGAATAACCCCATCTCTTTGTTTTGTT6720.1715668753382829No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTAAATTTCAT6620.16901379683622514No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG6410.16365233198190376No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC6370.16263110058108068No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT6330.16160986918025755No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA6140.15675902002634776No Hit
GTTTTAGGAAACTTCCTGTCAATCGGCCTATTGATTGGAAAGTATGTCAG6020.15369532582387843No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6020.15369532582387843No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT5940.15165286302223221No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5830.14884447666996864No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5820.14858916881976286No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG5770.14731262956873398No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT5680.14501485891688198No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAGACTCCTTCTTTTC5680.14501485891688198No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT5520.14092993331358952No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC5410.13812154696132595No Hit
GTGCCAACAGTTCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCA5380.13735562341070864No Hit
GTATATAATTGGAAGTTGGGGTACATTGCCACAGGATCATATTGTACAAA5360.13684500771029706No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC5360.13684500771029706No Hit
GGGTTGAGTGTCCCTGAACTGGAGCCACCAGCAGGAAAATACAGGCCCCT5300.1353131606090624No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG5160.13173885070618152No Hit
GTGGTCTCCATGCAACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC5150.13148354285597574No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT5130.1309729271555642No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5020.12816454080330061No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA4980.1271433094024775No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT4970.12688800155227173No Hit
ATTTGGAACAGGGTTGAGTGTCCCTGAACTGGAGCCACCAGCAGGAAAAT4960.12663269370206595No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4960.12663269370206595No Hit
ACTAGATACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGC4770.12178184454815616No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT4750.1212712288477446No Hit
GTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCGAGGACTGGGGA4740.12101592099753883No Hit
TATTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAG4710.12024999744692151No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4690.11973938174650994No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4680.11948407389630415No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4670.11922876604609839No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT4660.11897345819589261No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTTGTTGACA4650.11871815034568682No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4640.11846284249548106No Hit
ATTTAGTGCTGTTAGGGTAAAACCTAGCAGGCATAATTAATTTTAGTCTC4610.11769691894486373No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA4610.11769691894486373No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT4600.11744161109465794No Hit
GTCTAGAGGCAAATATTTAGTGCTGTTAGGGTAAAACCTAGCAGGCATAA4580.11693099539424638No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4560.11642037969383484No Hit
CTGTTGGCACTGTGGCCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAA4550.11616507184362905No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4540.11590976399342327No Hit
TCCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA4530.11565445614321748No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG4490.11463322474239439No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC4460.11386730119177704No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGGT4460.11386730119177704No Hit
CTCTTTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACACGTAGCGCC4440.1133566854913655No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATATTTAACCT4410.11259076194074814No Hit
CAGTTGGACCCTGCATTCGGAGCCAATTCAAACAATCCAGATTGGGACTT4340.11080360698930772No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA4320.11029299128889616No Hit
ACCTAGCAGCCATGGAAATGATGTATATTTCCGAGAAAGGACGACAGAAT4300.1097823755884846No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCGCG4280.10927175988807304No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4240.10825052848724992No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4210.10748460493663259No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA4200.10722929708642681No Hit
GGTCTCCATGCAACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAG4190.10697398923622103No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA4190.10697398923622103No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGGACACTCAACCCTGTTCCAA4150.10595275783539793No Hit
CTTTAATCCTGAATGGCAGACTCCTTCTTTTCCTAAAATTCATTTACATG4140.10569744998519214No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG4140.10569744998519214No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC4130.10544214213498637No Hit
ATTTACATACTCTTTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACA4110.1049315264345748No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG4060.10365498718354592No Hit
GGATTAAAGATAGGGACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC4050.10339967933334016No Hit
GAATTATCAGTCCCGATGAGTTTTGCTCCAGACCTGCTGCGAGCAAAACA3970.10135721653169391No Hit
TTCCCCCACTGTTTGGCTTTCAGCTATATGGATGATGTGGTACTGGGGGC3940.10059129298107658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)