FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506159
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16660.32914558468781546No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16520.3263796554047246No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14060.27777832657327045No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG13650.2696781051013614No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA13640.2694805387239978No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13310.26296084827099786No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG13310.26296084827099786No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12840.253675228534907No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12750.2518971311386343No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT12050.23806748472317987No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGAGCAGCA11990.23688208645899805No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA11730.23174536064754356No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11610.22937456411917995No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT11460.2264110684587254No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA11460.2264110684587254No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10670.2108033246469983No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10580.2090252272507256No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG10570.2088276608733619No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10260.20270310317508927No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGTGAGTCCAA10110.19973960751463474No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT9620.19005885502381664No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC9490.18749049211808935No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTAT9480.18729292574072573No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8950.17682190774045312No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8700.17188274830636224No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8680.17148761555163494No Hit
TCCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8670.17129004917427132No Hit
GGATCATATTGTACTAAAACTCAAGCAATGTTTCCGCAAACTGCCTGTAA8440.16674602249490772No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7690.15192854419263513No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7640.15094071230581696No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAA7590.1499528804189988No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7540.14896504853218062No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7430.1467918183811806No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7350.14521128736227154No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7280.1438283227207261No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7260.14343318996599883No Hit
AATCTGGATTGTTTGAGTTGGCTCCGAATGCAGGGTCCAACTGGTGATCG7240.14303805721127155No Hit
ATCCCGATTGGGACTTCAACCCCAACAAGGATCATTGGCCAGAGGCAAAT7220.14264292445654428No Hit
ATCCTAATCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7170.1416550925697261No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT7160.1414575261923625No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7040.13908672966399888No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC7020.13869159690927158No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCACGGCAACGGTCAGGTC6950.13730863226772616No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6950.13730863226772616No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6930.1369134995129989No Hit
GATTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6800.13434513660727163No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGT6710.1325670392109989No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC6640.13118407456945347No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACT6360.1256522160032717No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6220.1228862867201808No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATGTGG6150.12150332207863537No Hit
ATATTGTACTAAAACTCAAGCAATGTTTCCGCAAACTGCCTGTAAATAGA6120.12091062294654446No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6070.1199227910597263No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5930.11715686177663541No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5920.11695929539927176No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5790.11439093249354451No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5790.11439093249354451No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5660.11182256958781726No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5600.11063717132363546No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5600.11063717132363546No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5590.11043960494627184No Hit
GTACTAAAACTCAAGCAATGTTTCCGCAAACTGCCTGTAAATAGACCTAT5580.11024203856890818No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5560.10984690581418093No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5450.10767367566318092No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG5430.10727854290845368No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5400.10668584377636275No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5340.10550044551218096No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5330.10530287913481731No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5310.10490774638009005No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5290.1045126136253628No Hit
CCCCAAAAGACCGCCGTGTGGAGGGGTGAACCCTGGCCCGAATGCTCCCG5230.10332721536118097No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5230.10332721536118097No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5170.10214181709699917No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5100.10075885245545371No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5090.10056128607809009No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5090.10056128607809009No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5070.1001661533233628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)