FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532719
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTG40010.7510526187352056No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC30190.5667152851691042No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17440.3273770974941761No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA14810.2780077301541713No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA14520.2725639596109769No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC14190.26636932416527287No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG14070.2641167294577441No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13810.2592361075914319No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13090.24572053934625945No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12670.2378364578699089No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12570.23595929561363496No Hit
CCCTCATAAAACCAAACGTTGGGGTTACTCTCTTAACTTCATGGGATATG12040.22601033565538306No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG10950.20554926706199705No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10530.19766518558564644No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10310.19353542862184378No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG10000.18771622562739457No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9820.18433733356610146No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG9720.1824601713098275No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG9690.18189702263294533No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9150.17176034644906601No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9070.17025861664404687No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC8840.1659411434546168No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG8760.16443941364959763No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA8750.16425169742397022No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8720.16368854874708805No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC8610.1616236702651867No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8510.15974650800891277No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG8440.158432494429521No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8430.1582447782038936No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8410.15786934575263883No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC8390.15749391330138404No Hit
CTCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8350.15674304839887446No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8350.15674304839887446No Hit
ATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8330.15636761594761966No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8140.15280100766069915No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8020.15054841295317042No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA8000.15017298050191563No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7980.14979754805066087No Hit
GATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCT7950.14923439937377866No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7780.14604322353811297No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC7760.14566779108685818No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCAACCAATCGGCAGT7690.14435377750746642No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7640.14341519637932942No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA7520.1411626016718007No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7470.14022402054366373No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7470.14022402054366373No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA7270.13646969603111583No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT7240.13590654735423366No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA7140.13402938509795972No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT7110.13346623642107755No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7090.13309080396982273No Hit
AGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAA7090.13309080396982273No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7030.13196450661605838No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6970.130838209262294No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6960.13065049303666662No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC6930.13008734435978445No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6900.12952419568290224No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6900.12952419568290224No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6850.12858561455476528No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGAGCTTGGG6810.1278347496522557No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6800.12764703342662828No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTATGCCTGCTAGG6780.12727160097537352No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC6560.12314184401157084No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6520.12239097910906126No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6510.12220326288343386No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6480.12164011420655167No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6400.12013838440153252No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT6390.11995066817590512No Hit
CACACAATAGCTTGCCTGAGGGCTGTATGGTGAGGTGAACAATGTTCCGG6390.11995066817590512No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6370.11957523572465033No Hit
GTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6320.11863665459651337No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAACTTTAATCTCCTTTTTTCA6280.11788578969400378No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA6150.11544547876084765No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA6120.11488233008396546No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGG6110.11469461385833807No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6100.11450689763271069No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6020.11300516782769153No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC5970.11206658669955455No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC5970.11206658669955455No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCCCTCAGGCA5900.11075257312016279No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5890.11056485689453541No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5850.10981399199202582No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5830.10943855954077103No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGAGAG5820.10925084331514362No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5770.10831226218700667No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT5770.10831226218700667No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5770.10831226218700667No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5670.10643509993073272No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5640.10587195125385052No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5620.10549651880259574No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCTCTTAACTTCAT5610.10530880257696834No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5580.10474565390008615No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC5570.10455793767445876No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT5560.10437022144883137No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5560.10437022144883137No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATT5550.10418250522320398No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5530.1038070727719492No Hit
CCCTAATAAAACCAAACGTTGGGGTTACTCTCTTAACTTCATGGGATATG5500.103243924095067No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5450.10230534296693003No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5430.10192991051567524No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5430.10192991051567524No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5430.10192991051567524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)