FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532719
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTG23160.4347507785530458No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC20730.3891357357255889No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA14850.27875859505668094No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14680.2755674192210152No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA14240.26730790529340986No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC13420.2519151747919635No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG13330.25022572876131693No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13190.24759770160253342No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13020.24440652576686772No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12980.2436556608643581No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC11940.2241331733991091No Hit
CCCTCATAAAACCAAACGTTGGGGTTACTCTCTTAACTTCATGGGATATG11500.21587365947150372No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11200.21024217270268192No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11200.21024217270268192No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10830.2032966723544683No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG10810.20292123990321353No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10290.19315999617058902No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG9930.1864022120480028No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG9660.18133387395606312No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG9520.1787058467972796No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG9170.1721357789003208No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9090.17063404909530164No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9080.17044633286967426No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8870.16650429213149895No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG8830.1657534272289894No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8720.16368854874708805No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG8650.1623745351676963No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8450.15862021065514842No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA8310.15599218349636487No Hit
CTCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8150.15298872388632656No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA7900.1482958182456417No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC7890.1481081020200143No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7740.14529235863560339No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7700.1445414937330938No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT7680.144166061281839No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7640.14341519637932942No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC7490.14059945299491852No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7480.14041173676929114No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7450.13984858809240894No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA7370.1383468582873898No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7330.1375959933848802No Hit
GATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCT7320.1374082771592528No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7170.1345925337748419No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA6970.130838209262294No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTATGCCTGCTAGG6940.13027506058541183No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6870.12896104700602007No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6830.12821018210351048No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6770.1270838847497461No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC6770.1270838847497461No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT6700.12576987117035435No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6520.12239097910906126No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6520.12239097910906126No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCAACCAATCGGCAGT6510.12220326288343386No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6490.12182783043217905No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6470.12145239798092428No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA6450.1210769655296695No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6430.12070153307841469No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC6390.11995066817590512No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6360.11938751949902293No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAACTTTAATCTCCTTTTTTCA6310.11844893837088595No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT6310.11844893837088595No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6270.1176980734683764No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6240.11713492479149419No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC6230.11694720856586681No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC6220.11675949234023941No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAGGTCTTGCCC6200.11638405988898463No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6130.11507004630959285No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6110.11469461385833807No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6090.11431918140708329No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCCCTCAGGCA6040.11338060027894631No Hit
GGCCTCTACCGTCCCCTTCTTCTTCTGCCGTTCCAGCCGACCACGGGGCG5950.11169115424829977No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5890.11056485689453541No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC5880.110377140668908No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5830.10943855954077103No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5830.10943855954077103No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC5740.10774911351012448No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5730.10756139728449708No Hit
ATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5720.10737368105886969No Hit
GTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5670.10643509993073272No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5590.10493337012571356No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5560.10437022144883137No Hit
CACACAATAGCTTGCCTGAGGGCTGTATGGTGAGGTGAACAATGTTCCGG5560.10437022144883137No Hit
CTACCGTCCCCTTCTTCTTCTGCCGTTCCAGCCGACCACGGGGCGCACCT5540.10399478899757658No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCTCTTAACTTCAT5520.10361935654632179No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATT5480.10286849164381222No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTC5470.10268077541818482No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5470.10268077541818482No Hit
CCCTAATAAAACCAAACGTTGGGGTTACTCTCTTAACTTCATGGGATATG5460.10249305919255741No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC5440.10211762674130263No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5440.10211762674130263No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA5430.10192991051567524No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA5400.10136676183879308No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT5390.10117904561316565No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC5360.10061589693628348No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC5330.10005274825940129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)