FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559329
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTAAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT23250.41567664111819697No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG18400.32896559985268065No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC16350.29231454117344174No Hit
ATGTAAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC16030.2865934003064386No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC15390.27515111857243235No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15210.2719329768347431No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC15080.26960876335752304No Hit
CATTTAAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT14810.26478155075098914No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGAGAAAGGCATTAAACCTTAT14480.25888162423189215No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC13400.23957277380575653No Hit
CTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT13260.23706977467644266No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13220.23635463206806728No Hit
TATTTACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCGTCC13020.23277891902619033No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT12660.2263426355508118No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG12360.22097906598799633No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTCAA11460.20488835729955002No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11400.2038156433869869No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT10970.19612786034695143No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10700.19130064774041752No Hit
ATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATTCT10430.18647343513388365No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10340.184864364265039No Hit
CTGTAAGAGGGCCCACATATTGTTCACATCTATTAATAATGTCCTCCTGT9900.17699779557290968No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9640.17234936861846964No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9600.17163422601009423No Hit
GTTTTCGGAAATTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAA9310.16644944209937265No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8900.15911923036352485No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8890.158940444711431No Hit
CCTCCTTCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGG8850.15822530210305563No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8760.15661623123421098No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8580.15339808949652173No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA8570.1532193038444279No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8020.14338609297926622No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8000.14302852167507854No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG7730.1382013090685446No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7730.1382013090685446No Hit
CTCCCATAGGAATCTTGCGAAAGCCCATGATGATGGGATGGGAATACAAG7540.13480438167876152No Hit
GGAATGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTCCCGATA7460.13337409646201073No Hit
CTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTA7440.13301652515782303No Hit
GTTGTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG7390.1321225968973538No Hit
CCTCCGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT7190.12854688385547683No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7140.12765295559500758No Hit
GTTGACAACAGTGCCAACAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7000.1251499564656937No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC6900.12336209994475525No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6860.12264695733637986No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6720.12014395820706596No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT6700.11978638690287827No Hit
GCCTACGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6650.11889245864240904No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCGTCC6650.11889245864240904No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6610.11817731603403364No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTGACAC6590.11781974472984594No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6590.11781974472984594No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC6540.11692581646937669No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6540.11692581646937669No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6520.116568245165189No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGAGAAAGGCATTAAAC6490.11603188820890746No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG6460.1154955312526259No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6290.1124561751670305No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6190.110668318646092No Hit
GTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTGTGG6180.11048953299399816No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6120.10941681908143508No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6090.10888046212515354No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6090.10888046212515354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6060.10834410516887198No Hit
CTTGTATTCCCATCCCATCATCATGGGCTTTCGCAAGATTCCTATGGGAG6050.10816531951677813No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6020.10762896256049659No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5990.10709260560421506No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTGACAC5960.1065562486479335No Hit
GCTAGATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTT5880.10512596343118272No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5850.10458960647490119No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5690.1017290360413996No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5630.10065632212883653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)