FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498729
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA21250.42608310324845755No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC19650.3940015519450443No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15400.3087849312953528No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14510.2909395683828292No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATCAAACCGTAT14310.28692937446990247No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA13650.2736957345572445No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT12400.2486320226014529No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12130.24321826081900189No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12030.24121316386253858No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT11970.24001010568866057No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC11860.2378044990365509No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG11590.23239073725409995No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC11450.22958360151505128No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC11310.2267764657760026No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA11080.22216474277613696No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA11030.2211621942979053No Hit
GGTTAGGATAGAACCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10720.21494639373286895No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10540.21133721921123497No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10170.20391836047232065No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10060.20171275382021098No Hit
CTATTAATAATGTCCTCCCGCAAATGAATGTGAGGAAAGGAGGGAGTTTG9900.19850459868986964No Hit
GACGCAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG9430.189080642994492No Hit
AGTCTAGACTCTGAGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9220.184869939385919No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG8910.17865413882088269No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA8910.17865413882088269No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTCACAGTTAATGAAAAA8810.17664904186441938No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG8540.17123528008196837No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC8280.1660220279951637No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7990.16020724682142004No Hit
GATTCGTCCCCATGCCTTGTCGAGGTTTGGAAGACCAACCTCCCATGCTG7860.15760062077801773No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA7730.1549939947346154No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATCAAAC7570.15178583960427405No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7350.14737462630005474No Hit
GGATAGAACCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7330.14697360690876207No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7250.1453695293435914No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6990.14015627725678675No Hit
ATATGGTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6980.13995576756114042No Hit
CTCCAAAAGACACTAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6900.13835168999596975No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6850.13734914151773808No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6810.13654710273515275No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6790.1361460833438601No Hit
ATACATAGCAGAGGCGGTGTCGAGGAGATCTCGAACAGAAGGAAAGAAGT6680.13394047669175044No Hit
GGATAATACGGTTTGATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6650.13333894760481144No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6610.1325369088222261No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6490.1301307924744701No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6440.12912824399623843No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTGTTCC6380.12792518582236045No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC6360.1275241664310678No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCC6330.12692263734412876No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACAAAATGCC6280.12592008886589712No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCGGG6230.12491754038766545No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6160.12351397251814111No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6120.12271193373555578No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6090.12211040464861678No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6060.12150887556167779No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6010.12050632708344611No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5960.11950377860521445No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5890.11810021073569012No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5860.11749868164875113No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5710.11449103621405612No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5690.11409001682276346No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5610.11248593925759282No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5580.11188441017065381No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5550.1112828810837148No Hit
GATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATTGGAGGAC5510.11048084230112946No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTA5500.11028033260548314No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5430.10887676473595881No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAAGGGAT5430.10887676473595881No Hit
ATATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG5370.10767370656208082No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTC5350.10727268717078814No Hit
GTTCAGGATAATACGGTTTGATGCCTTTGTCCAAGGGCAAATATTTGGTA5280.1058691193012638No Hit
CTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG5210.1044655514317395No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC5210.1044655514317395No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5210.1044655514317395No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5190.10406453204044681No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5170.10366351264915416No Hit
ATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTTGTACAATATGGT5130.10286147386656883No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5110.10246045447527614No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5100.10225994477962982No Hit
CTGTGAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCCGC5100.10225994477962982No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5090.10205943508398349No Hit
GCTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5060.10145790599704449No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5050.10125739630139816No Hit
GCGTTCTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT5030.10085637691010549No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT5020.10065586721445915No Hit
GGACAAATCTTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAG5020.10065586721445915No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5010.10045535751881284No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5010.10045535751881284No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5010.10045535751881284No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4990.10005433812752015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)