FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499539
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGACCTTGGGCAAGACTTGGTGGGCGTTCACGGTGGTTTCCATGC19470.3897593581281942No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG16020.32069568141826765No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15780.3158912517340988No Hit
GCACAGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG15470.30968553005871413No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14440.289066519330823No Hit
GTCCTGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC14100.2822602439449172No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13450.2692482468836267No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12540.25103145099782No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT12460.2494299744364304No Hit
ATTTACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA11370.22760985628749708No Hit
GTCTATAAGGGGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC11210.22440690316471787No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGATTGAAGTCCCAATCTGGA10770.21559878207707506No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG10370.20759139927012704No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9860.19738198619126834No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG9690.19397884849831545No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9600.19217718736675216No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTGCCAATTACATATCCCATGAA9520.19057571080536254No Hit
GTCGAGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATC9430.18877404967379924No Hit
CTTCCTTTTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGG9100.18216795885806714No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA8620.17255909948972953No Hit
TTTACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACAC8410.16835522351608181No Hit
CTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG8370.16755448523538705No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC8360.16735430066521334No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG8070.16154894813017603No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT7900.15814581043722312No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATAGATGAGAGTCCAA7840.1569447030161809No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT7650.1531411961828806No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7620.15254064247235952No Hit
CTATATAAGAGAGAAACTACACGAAGCGCTTCATTTTGTGGGTCACCATA7510.1503386122004488No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7390.14793639735836442No Hit
GGATTAAAGACAGGTACAGTGGAAGAATAAAGCCCAGTAAAGTTTCCCAC7310.1463349207969748No Hit
CACATATTGTTGACATCTATCAATAATGTCCTCCTGTAAATGAATGTTAG7300.14613473622680112No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCA7200.14413289052506412No Hit
GTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC7200.14413289052506412No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7030.1407297528321112No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7020.14052956826193752No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7010.1403293836917638No Hit
CTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT6990.1399290145514164No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG6900.1381273534198531No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6770.135524954007595No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCATCTATGTCAACGACCGACCT6750.1351245848672476No Hit
TCCCATGGCTGCTCGGATGTACTGCCAACTGGATCCTGCGCGGGACGTCC6580.13172144717429468No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACTTTACCACAG6570.131521262604121No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6570.131521262604121No Hit
ATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATTTACAT6550.1311208934637736No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6520.1305203397532525No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG6430.1287186786216892No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6350.12711720206029958No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA6310.1263164637796048No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6240.1249151717883889No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6220.1245148026480415No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6210.1243146180778678No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6200.1241144335076941No Hit
CTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT6180.12371406436734671No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6170.12351387979717299No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCCACTG6160.12331369522699931No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6130.12271314151647819No Hit
CTCTTATATAAGACCTTGGGCAAGACTTGGTGGGCGTTCACGGTGGTTTC6080.1217122186656097No Hit
CTTTAAAACTAGGCATTATTTACATACTCTGTGGAAGGCGGGCATTCTAT6030.12071129581474119No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6010.12031092667439379No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGTTGAGTTGATGA5960.1193100038235253No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5900.1181088964024831No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5900.1181088964024831No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5870.11750834269196198No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5830.11670760441126718No Hit
ATATAAGAGAGAAACTACACGAAGCGCTTCATTTTGTGGGTCACCATATT5820.11650741984109349No Hit
TTGTACACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGTTG5800.11610705070074609No Hit
GTTGAACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC5790.11590686613057238No Hit
CCTGCCCCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5780.11570668156039869No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCACGGCAACGGTCAGGTC5740.1149059432797039No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5720.11450557413935648No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5710.11430538956918278No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT5680.11370483585866169No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCTGTG5660.11330446671831429No Hit
CACAAATTGTTGACATCTATCAATAATGTCCTCCTGTAAATGAATGTTAG5660.11330446671831429No Hit
GTGTTGGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAGGTAATTTGG5550.1111024364464036No Hit
GCCAATTACATATCCCATGAAGTTAAGGGAGTAACCCCAACGTTTGGTTT5550.1111024364464036No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG5520.11050188273588249No Hit
AGGCCAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACTCCACCT5500.11010151359553508No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5360.10729892961310328No Hit
GTACGGGGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA5320.10649819133240848No Hit
GCTTAATTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTGCCA5290.10589763762188739No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5280.10569745305171367No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT5210.10429616106049779No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5200.10409597649032407No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5200.10409597649032407No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAAT5150.10309505363945558No Hit
AACTTAAGCAATGTTTTCGGAAATTGCCTGTAAATCGACCTATTGATTGG5150.10309505363945558No Hit
CTGTTCAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5140.10289486906928187No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5110.10229431535876078No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGTTGAGTTGATGAATCTG5100.10209413078858708No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5050.10109320793771857No Hit
ACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGA5030.10069283879737118No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5030.10069283879737118No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5020.10049265422719747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)