FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418947
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG17020.4062566386678983No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG13180.31459826660651585No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12550.2995605649401953No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11390.271872098379986No Hit
GTATACATGCATACAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA10700.25540223465020634No Hit
CTATATAAGAGAGAAACTACACGCAGCGCATCATTCTGTGGGTCACCATA10690.2551635409729632No Hit
ATTTACATACTATGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA10490.2503896674280995No Hit
CTTATATAGAATGCCAGCCTTCCACATAGTATGTAAATAATGCCTAGTTT10160.24251277607907443No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10030.23940975827491306No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG9910.23654543414799484No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG9900.23630674047075165No Hit
CTCCTACTGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9820.2343971910528062No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9150.2184047146775129No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8660.20670872449259692No Hit
GGATCATATTATACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAA8550.20408309404292188No Hit
ATTGACAACAGTGCCAGCTGCGCCTCCTACTGCCTCCACCAATCGGCAGT8450.20169615727049006No Hit
ATATAAGAGAGAAACTACACGCAGCGCATCATTCTGTGGGTCACCATATT8310.19835444578908548No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8180.1952514279849241No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG7900.188568005022115No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7760.18522629354071038No Hit
CCTACTGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7670.18307805044552175No Hit
GGGTTCACCCCTCCACACGGAGGTCTTTTGGGGTGGAGCCCTCAGGCTCA7660.1828393567682786No Hit
TTTACATACTATGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7530.17973633896411717No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT7050.16827904245644437No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7030.16780165510195802No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG7010.16732426774747164No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6980.16660818671574207No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT6860.16374386258882387No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6820.16278908787985116No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6820.16278908787985116No Hit
ACTGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC6630.15825390801223066No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6580.15706043962601474No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6550.1563443585942852No Hit
ATATTATACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA6530.1558669712397988No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6510.15538958388531246No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6480.15467350285358292No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACTGCATACAGACCACCAAATGCC6420.1532413407901238No Hit
GTATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA6410.1530026471128806No Hit
CCTTATGAGTCCAAGGGGTACTAACATTGAGATTCCCGAGATTGAGATCT6380.15228656608115107No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTGATGCC6300.1503770166632056No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC6240.14894485459974652No Hit
GATATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTG6170.14727399885904421No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6040.14417098105488282No Hit
TCCACATAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA6010.14345490002315328No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6010.14345490002315328No Hit
ATCCTAAACTTACTAAATATTTGCCCTTGGATAAAGGCATCAAACCTTAT5980.14273881899142374No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGGAGAGGACAAACGGGCAAC5940.141784044282451No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5900.14082926957347827No Hit
CTCCAAAAGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTGAGA5850.13963580118726235No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCTGGTC5740.1370101707375873No Hit
CTATGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCA5680.13557800867412823No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5570.13295237822445322No Hit
GCTTAGATTGTATACATGCATACAAAGGCATCAGAGCAGGATAGCCACAT5500.1312815224837509No Hit
CCCCAAAAGACCTCCGTGTGGAGGGGTGAACCCTGGCCCGAATGCTCCCG5470.13056544145202137No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5360.12793981100234636No Hit
CTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACT5310.12674634261613046No Hit
CTCTTATATAGAATGCCAGCCTTCCACATAGTATGTAAATAATGCCTAGT5300.12650764893888725No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTTCTG5180.12364332481196905No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5050.12054030700780767No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5050.12054030700780767No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5010.11958553229883494No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTGTATGCATGTATA5000.11934683862159176No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTGATTATGCCTGCTAGG4990.11910814494434857No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4990.11910814494434857No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4980.1188694512671054No Hit
CAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAACC4890.11672120817191674No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4780.11409557772224171No Hit
ATCCTGCTCTGATGCCTTTGTATGCATGTATACAATCTAAGCAGGCTTTC4740.11314080301326897No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4710.11242472198153942No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4710.11242472198153942No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4700.11218602830429625No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4700.11218602830429625No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4690.11194733462705306No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA4690.11194733462705306No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC4690.11194733462705306No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG4670.1114699472725667No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4670.1114699472725667No Hit
GCTTTAGGGCATGGACATCGACCCGTATAAAGAATTTGGAGCTTCTGTGG4590.10956039785462122No Hit
GATTAAGATTGATTATGCCTGCTAGGTTCTATCCTAAACTTACTAAATAT4520.10788954211391893No Hit
GTCCAAGGGGTACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4520.10788954211391893No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT4500.10741215475943257No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4410.10526391166424391No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4410.10526391166424391No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4400.10502521798700074No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4400.10502521798700074No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4370.10430913695527119No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA4370.10430913695527119No Hit
CTTTAAAACTAGGCATTATTTACATACTATGTGGAAGGCTGGCATTCTAT4350.10383174960078483No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4330.10335436224629846No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGGT4310.1028769748918121No Hit
ATCCTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGA4300.1026382812145689No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4300.1026382812145689No Hit
ATATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG4270.10192220018283935No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4230.10096742547386663No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTAAT4230.10096742547386663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)