FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541670
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA22720.41944357265493754No Hit
CTATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATA16100.2972289401295992No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14480.2673214318681116No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT13810.25495227721675556No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12970.23944468034042868No Hit
ATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATATT12870.23759853785515167No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG12610.2327985673934314No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG12540.23150626765373752No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG12010.22172171248176936No Hit
CTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT11080.20455258736869314No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA10710.19772186017316817No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA10260.1894142189894216No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC10150.1873834622556169No Hit
CGCTCGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCT9670.17852197832628725No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9340.17242970812487307No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAAC9310.17187586537928998No Hit
CTAACATTGGGATTCCCGAGATTGAGTTCTTCTGCGACGCGGCGATTGAG9290.17150663688223455No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8870.16375283844407112No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC8660.15987593922498938No Hit
CATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT8640.159506710727934No Hit
ATATTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGCTGACTACT8620.15913748223087856No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8620.15913748223087856No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8620.15913748223087856No Hit
GGATACTAACATTGGGATTCCCGAGATTGAGTTCTTCTGCGACGCGGCGA8550.15784518249118468No Hit
GTGTTCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAGTTGGCTC8470.15636826850296304No Hit
CGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCT8330.15378366902357524No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG8210.15156829804124283No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC8130.1500913840530212No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8120.14990676980449352No Hit
GACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTA8120.14990676980449352No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8000.1476913988221611No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA7830.14455295659719017No Hit
CAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG7810.14418372810013477No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7780.14362988535455168No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7700.14215297136633004No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT7680.14178374286927467No Hit
TACATAGATCATTAACCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAA7530.13901452914135914No Hit
GCATCAAACCTTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT7470.13790684365019293No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC7410.1367991581590267No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7360.1358760869163882No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7310.1349530156737497No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTCTGACATACT7210.13310687318847267No Hit
GAGACACTGTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACT7140.13181457344877878No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7110.13126073070319566No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCA7100.131076116454668No Hit
CTACTATTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG7090.13089150220614026No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA7070.13052227370908487No Hit
CAAGGATCACTGGCCAGAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGC6950.12830690272675246No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTG6860.12664537449000315No Hit
GGTGAACCCTGGCCCGAATGCTCCCGCTCCTACCTGATTTGCCTCTGGCC6860.12664537449000315No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTATA6790.12535307475030924No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC6790.12535307475030924No Hit
CATCGAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT6740.12443000350767072No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG6740.12443000350767072No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTG6620.12221463252533832No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6570.12129156128269981No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6530.12055310428858898No Hit
CTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6460.1192608045488951No Hit
CAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAATATTTGT6300.11630697657245186No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG6290.11612236232392416No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6270.11575313382686876No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAATT6240.11519929108128564No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6190.11427621983864714No Hit
GTCTAGCCTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCG6130.11316853434748095No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6100.11261469160189783No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6070.11206084885631473No Hit
CCGCACGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG6060.11187623460778703No Hit
GGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG5990.1105839348680931No Hit
ACATCGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5950.1098454778739823No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5820.1074454926431222No Hit
GTGACCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAGTTGGCTC5820.1074454926431222No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC5770.1065224214004837No Hit
GTAAATTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACACGCATATA5770.1065224214004837No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5720.10559935015784518No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTGTCTCTTCCAAAAGTAAGA5630.10393782192109587No Hit
CTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5600.10338397917551277No Hit
CCTTATGAGTCCAAGGGATACTAACATTGGGATTCCCGAGATTGAGTTCT5550.10246090793287427No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5550.10246090793287427No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAATTGGCGA5520.10190706518729115No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG5500.10153783669023576No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5490.10135322244170805No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG5440.10043015119906955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)