FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355184
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20050.5644961484751566No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18200.5124104689400423No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG15140.42625793954682645No Hit
ATATAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTTTCCATGC14000.394161899184648No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG12480.35136717870174333No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10390.2925244380377494No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG10360.29167980539663946No Hit
TCCAAATTACCTCCCACCCAGGTGGCCAGATTCATCAACTCACCCCAACA10290.28970899590071625No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10020.2821073021307266No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9320.26239920717149423No Hit
GTCAACGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCTTTTACACAATGT8800.24775890805892156No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8720.245506554349295No Hit
AACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT8640.24325420063966846No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG8510.2395941258615253No Hit
GTGTAAGAGGGCCCACATATTGTTGACATCTGTTAATAATGTCCTCCTGT8510.2395941258615253No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8220.231429343664129No Hit
CTATATAGGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7870.22157529618451283No Hit
CTCTCAGAGGTGGAGAGATGGGCGTAGGCTGTCTTCCTGACTGCCGATTG7600.2139736024145232No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7390.20806117392675347No Hit
GTAATCTGGAAGACCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAAT7340.20665345285823686No Hit
ATCCTGATGATGTGTTCTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAG7170.20186720122528043No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG7160.2015856570115771No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7150.2013041127978738No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG7110.2001779359430605No Hit
GTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT7070.1990517590882472No Hit
ATTTCCGAGAGAGGACAACAGAGTTGTCAGTCCCGATAAGTTTCGCTCCA7030.19792558223343396No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTCAA6850.19285778638677417No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6800.19145006531825756No Hit
GCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCC6760.1903238884634443No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6760.1903238884634443No Hit
CCTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6710.1889161673949277No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6690.18835307896752107No Hit
CTCTTACACCTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6380.17962520834271814No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6340.17849903148790486No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6290.17709131041938825No Hit
GTCCCGTGCTGGTTGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6210.1748389567097617No Hit
ATATAGGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6050.17033424929050858No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6030.16977116086310196No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5970.16808189558088202No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5870.16526645344384883No Hit
ATTGACAACAGTGCCAGCAGCCCCTCCTCCTGCTTCCACCAATCGGCAGT5860.1649849092301455No Hit
CTACTGTACCTGTCTTCAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5780.16273255552051893No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAGGAGAGAAACTACA5570.15682012703274922No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT5520.15541240596423261No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5480.15428622910941933No Hit
CTCTTATATAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTTTC5380.15147078697238614No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5370.15118924275868284No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC5310.1494999774764629No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTCAACAATACC5290.14893688904905625No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5230.14724762376683634No Hit
GTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT5210.14668453533942968No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5200.14640299112572638No Hit
CTCGAAAATACACCTCCTTCCCATGGCTGCTCGGATGTGCTGCCAACTGG5170.14555835848461643No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5120.14415063741609982No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC5110.1438690932023965No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5050.14217982792017658No Hit
TACCTTGATAGTCCAGAAGAGCCAACAAGAAGATGAGGCATAGCAGCAGG4970.13992747421055002No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4900.13795666471462678No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4820.13570431100500022No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4810.1354227667912969No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4770.13429658993648363No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTAT4760.1340150457227803No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA4750.13373350150907698No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGA4740.13345195729537368No Hit
GCCTATAGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4720.13288886886796702No Hit
GAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGT4720.13288886886796702No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4690.13204423622685707No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4660.13119960358574712No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGGCCTGGTGGGCGTTC4610.1297918825172305No Hit
ATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTA4600.12951033830352718No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4570.12866570566241722No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATAT4540.12782107302130727No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4530.12753952880760394No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4530.12753952880760394No Hit
GCAGACGAAGAAGGGGACGATAAAGGCCCAAACGGCCCCGAGACGGGTCG4480.12613180773908733No Hit
GGATTGAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4410.12416099824316411No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4390.12359790981575747No Hit
ATACAGCACTCAGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGAATCTG4380.12331636560205415No Hit
GTCCAGAAGAGCCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4370.12303482138835081No Hit
GGCTATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGA4350.12247173296094419No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGCGCTTCTGTGGA4350.12247173296094419No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4330.12190864453353754No Hit
CTCTCAGAGGTGGAGAGATGGGCGTAGGCTGTCTTCCCGACTGCCGATTG4300.12106401189242759No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4300.12106401189242759No Hit
CTCACCTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGG4290.12078246767872428No Hit
GCACTCAGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGAATCTGGCCAC4240.11937474661020767No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA4230.11909320239650435No Hit
GCTATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT4220.11881165818280104No Hit
CAAATGTTCCACGCATGCGCCGATGGCCTATAGCCAAGCCCCATCCAGTG4220.11881165818280104No Hit
GGAACATATTGTACTAAAAATCAAACAATGTTTTCGGAAACTGCCTGTAA4200.11824856975539438No Hit
CTTCCCGACTGCCGATTGGTGGAAGCAGGAGGAGGGGCTGCTGGCACTGT4200.11824856975539438No Hit
GATTGAATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4180.11768548132798774No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4170.11740393711428443No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACTCC4120.11599621604576782No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAAATTCCTATGGGAG4080.11487003919095455No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4000.11261768548132799No Hit
ACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAAC3930.11064687598540475No Hit
ATCTTGAGTCCCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGT3920.11036533177170144No Hit
TTGTACACCTCACCATACAGCACTCAGGCAAGCTATTCTTTGTTGGGGTG3900.1098022433442948No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG3830.10783143384837156No Hit
GTATGGTGAGGTGTACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG3810.10726834542096492No Hit
ATTCGTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG3800.1069868012072616No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC3780.10642371277985495No Hit
CTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACTCCT3760.10586062435244831No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA3740.10529753592504168No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3720.10473444749763502No Hit
CCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTATAT3710.10445290328393173No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAGGAGAGAAACTACAC3710.10445290328393173No Hit
CGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAACA3700.10417135907022838No Hit
GGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGA3640.10248209378800846No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT3630.10220054957430515No Hit
CTTCAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG3630.10220054957430515No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC3620.10191900536060183No Hit
AACTTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT3620.10191900536060183No Hit
GGTCTTGCATGGTCCCGTGCTGGTTGTTGATGTTCCTGGAAGTAGAGGAC3610.1016374611468985No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTG3590.10107437271949188No Hit
CTCCTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3590.10107437271949188No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACAACC3570.10051128429208524No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3570.10051128429208524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)