FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355184
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18920.5326816523266813No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16240.45722780305419164No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG15620.43977206180458583No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG13090.36854137573764584No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG12950.36459975674579936No Hit
ATATAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTTTCCATGC12140.3417946754358304No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11160.3142033424929051No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG10210.2874566421910897No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9740.27422406414703365No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9380.26408847245371414No Hit
GTGTAAGAGGGCCCACATATTGTTGACATCTGTTAATAATGTCCTCCTGT8990.2531082481192847No Hit
TCCAAATTACCTCCCACCCAGGTGGCCAGATTCATCAACTCACCCCAACA8790.24747736384521826No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8640.24325420063966846No Hit
GTCAACGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCTTTTACACAATGT8540.24043875850263524No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG8230.23171088787783237No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7670.21594441191044642No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7540.21228433713230327No Hit
GTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT7500.21115816027749No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7410.2086242623541601No Hit
CTATATAGGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7300.2055272760034236No Hit
CCTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7280.20496418757601692No Hit
ATCCTGATGATGTGTTCTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAG6990.19679940537862067No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG6780.19088697689085093No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6690.18835307896752107No Hit
ATTTCCGAGAGAGGACAACAGAGTTGTCAGTCCCGATAAGTTTCGCTCCA6620.1863822694715978No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6580.18525609261678455No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTCAA6510.18328528312086131No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6480.18244065047975133No Hit
CTCTTACACCTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6430.18103292941123475No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6320.1779359430604982No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6320.1779359430604982No Hit
GTCCCGTGCTGGTTGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6290.17709131041938825No Hit
GTAATCTGGAAGACCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAAT6240.17568358935087167No Hit
CTCTCAGAGGTGGAGAGATGGGCGTAGGCTGTCTTCCTGACTGCCGATTG5950.1675188071534754No Hit
ATATAGGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5920.1666741745123654No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5790.16301409973422226No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5690.16019865759718904No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC5680.15991711338348574No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAGGAGAGAAACTACA5590.15738321546015585No Hit
ATTGACAACAGTGCCAGCAGCCCCTCCTCCTGCTTCCACCAATCGGCAGT5590.15738321546015585No Hit
GTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT5590.15738321546015585No Hit
CTACTGTACCTGTCTTCAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5560.1565385828190459No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC5490.15456777332312266No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT5430.15287850804090275No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGCGCTTCTGTGGA5430.15287850804090275No Hit
GCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCC5410.1523154196134961No Hit
CTCGAAAATACACCTCCTTCCCATGGCTGCTCGGATGTGCTGCCAACTGG5360.15090769854497948No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5300.14921843326275958No Hit
GAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGT5230.14724762376683634No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTCAACAATACC5230.14724762376683634No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4960.1396459299968467No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4920.13851975314203344No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA4850.13654894364611017No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4850.13654894364611017No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA4840.13626739943240687No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4800.1351412225775936No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4720.13288886886796702No Hit
GGATTGAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4700.13232578044056037No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4680.13176269201315374No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4640.13063651515834046No Hit
ATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTA4640.13063651515834046No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4630.13035497094463713No Hit
CTTCATCTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTTTACGCGGT4600.12951033830352718No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4600.12951033830352718No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTAT4500.126694896166494No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4500.126694896166494No Hit
CAAATGTTCCACGCATGCGCCGATGGCCTATAGCCAAGCCCCATCCAGTG4450.12528717509797738No Hit
CTCTTATATAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTTTC4430.12472408667057074No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4400.12387945402946078No Hit
GCACTCAGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGAATCTGGCCAC4390.12359790981575747No Hit
GCTATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT4370.12303482138835081No Hit
CTCACCTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGG4370.12303482138835081No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4330.12190864453353754No Hit
ATTCGTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG4270.12021937925131762No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGA4260.11993783503761431No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4240.11937474661020767No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAAATTCCTATGGGAG4200.11824856975539438No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACTCC4200.11824856975539438No Hit
TACCTTGATAGTCCAGAAGAGCCAACAAGAAGATGAGGCATAGCAGCAGG4190.11796702554169108No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4170.11740393711428443No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAGGAGAGAAACTACAC4160.11712239290058112No Hit
GCCTATAGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4150.11684084868687779No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4150.11684084868687779No Hit
ACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAAC4150.11684084868687779No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4140.11655930447317447No Hit
CTTCCCGACTGCCGATTGGTGGAAGCAGGAGGAGGGGCTGCTGGCACTGT4100.11543312761836119No Hit
GTATGGTGAGGTGTACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG4090.11515158340465786No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGGCCTGGTGGGCGTTC4030.11346231812243794No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATAT4020.11318077390873463No Hit
ATCTTGAGTCCCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGT4010.11289922969503131No Hit
GGAACATATTGTACTAAAAATCAAACAATGTTTTCGGAAACTGCCTGTAA3990.11233614126762467No Hit
CTCTCAGAGGTGGAGAGATGGGCGTAGGCTGTCTTCCCGACTGCCGATTG3970.11177305284021802No Hit
GTCCAGAAGAGCCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3960.11149150862651472No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3960.11149150862651472No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3940.11092842019910806No Hit
GGCTATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGA3910.11008378755799811No Hit
CTCCTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3900.1098022433442948No Hit
CTTCAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG3890.10952069913059148No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTG3880.10923915491688814No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3860.10867606648948151No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3810.10726834542096492No Hit
GAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCAGCT3680.10360827064282176No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3680.10360827064282176No Hit
GCAGACGAAGAAGGGGACGATAAAGGCCCAAACGGCCCCGAGACGGGTCG3660.10304518221541512No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG3660.10304518221541512No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3660.10304518221541512No Hit
ATACAGCACTCAGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGAATCTG3630.10220054957430515No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3620.10191900536060183No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3590.10107437271949188No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC3590.10107437271949188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)