FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513587
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG18030.351060287740928No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17620.34307721963367455No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT17620.34307721963367455No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16970.33042113604900436No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG16510.3214645230506224No Hit
CCTCCCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15810.3078348945748237No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC14930.2907005044909626No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA14550.2833015633183862No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13820.2690878079079104No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA12570.24474918562969855No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT12550.24435976767324719No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11980.23326135591438257No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA11820.23014601226277143No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11630.22644654167648326No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10680.20794918874504223No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT10330.20113437450714292No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10190.19840844881198316No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA9750.1898412537700526No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG9630.18750474603134423No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9580.18653120114021576No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9570.18633649216199008No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9140.1779640060982852No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA9040.17601691631602823No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA8880.17290157266441714No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAAG8740.17017564696925738No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTTTTGCCTTCTG8690.1692021020781289No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG8690.1692021020781289No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8540.1662814674047435No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8470.16491850455716364No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC8400.16355554170958378No Hit
GTATACATGCGTATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA8260.16082961601442405No Hit
TAGCAGCAGGATGAAGAGGAATATGATAAAACGCCGCAGACACATCCAGC8240.16044019805797263No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC8110.15790898134103862No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG8100.1577142723628129No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG8080.15732485440636154No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7980.15537776462410458No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA7750.1508994581249136No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCATATTCATTTACAGG7500.14603173366927122No Hit
TCCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCC7500.14603173366927122No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA7500.14603173366927122No Hit
CTATTGTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT7270.14155342717008024No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7180.13980104636604898No Hit
ATATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG7170.1396063373878233No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7080.13785395658379204No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6780.1320126872370212No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6740.1312338513241184No Hit
ATGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCC6690.13026030643298994No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTGAGGGAG6660.12967617949831284No Hit
CATGTATACAAGCGAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6630.12909205256363576No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT6550.1275343807378302No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA6550.1275343807378302No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6520.1269502538031531No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6500.12656083584670172No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6450.12558729095557325No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCCTACAGACCACCAAATGCC6430.12519787299912186No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6420.12500316402089617No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6410.12480845504267046No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6400.12461374606444478No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6270.12208252934751075No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6260.12188782036928504No Hit
CTGCAGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTAT6160.11994073058702809No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC6120.11916189467412533No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATACGCATGTATA6120.11916189467412533No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCTCT6080.11838305876122254No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6030.11740951387009407No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5990.11663067795719129No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5960.1160465510225142No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA5930.11546242408783712No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5910.11507300613138571No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA5890.11468358817493433No Hit
CTTTAAAACTAGGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTAT5860.11409946124025724No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5820.11332062532735446No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5780.1125417894144517No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAT5750.11195766247977461No Hit
GTATACAAGCGAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT5720.1113735355450975No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5620.10942644576284057No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5570.10845290087171208No Hit
ACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG5540.10786877393703502No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5540.10786877393703502No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5460.10631110211122945No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5440.10592168415477807No Hit
CCTTTATACGCATGTATACAAGCGAAGCAGGCTTTCACTTTCTCGCCAAC5410.10533755722010098No Hit
GGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC5390.1049481392636496No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5360.1043640123289725No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5320.10358517641606971No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5320.10358517641606971No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5300.10319575845961833No Hit
GGGTTCACCCCTCCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA5200.10124866867736139No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC5170.10066454174268429No Hit
ATCCTGCTCTGATGCCTTTATACGCATGTATACAAGCGAAGCAGGCTTTC5160.1004698327644586No Hit
CCTGTAAATGAATATGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG5160.1004698327644586No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT5160.1004698327644586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)